SUN Huijuan,GAO Lei.Planting Adaptability of Different Ganoderma lucidumStrains in Lasa[J].HEILONGJIANG AGRICULTURAL SCIENCES,2024,(01):46-51.[doi:10.11942/j.issn1002-2767.2024.01.0046]
不同灵芝菌株在拉萨种植适应性研究
- Title:
- Planting Adaptability of Different Ganoderma lucidumStrains in Lasa
- 文章编号:
- 8
- Keywords:
- Ganoderma lucidum; Lasa; agronomic trait; active ingredients
- 文献标志码:
- A
- 摘要:
- 为筛选获得适宜西藏地区栽培的优质灵芝菌株,以6个不同灵芝菌株为试材,采用袋料栽培,比较分析灵芝菌株主要农艺性状及子实体活性成分含量。多基因测序和聚类分析结果显示菌株15和26均为白肉灵芝;菌株26 子实体平均单株质量最高,为82.01 g,菌盖直径最大,为16.00 cm,生物学效率最高,为23.60%;菌株15子实体中三萜含量最高,为1.91%,比药典高出282.00%,菌株26次之,为1.88%,灵芝菌株15子实体中多糖含量最高,为1.27%,菌株26次之,为1.13%,菌株15和26的多糖和三萜含量显著高于其他灵芝菌株,结合主要农艺性状、生物学效率和活性成分含量来判断,白肉灵芝15和26均适宜拉萨地区种植推广。
- Abstract:
- In order to select and obtain the high qualityGanoderma lucidumstrains suitable for cultivation in Xizang, the main agronomic characters and active components in fruit bodies of 6 differentGanoderma lucidumstrains were compared and analyzed by using bag material cultivation. The results showed that strains 15 and 26 were allGanoderma lucidum.The average single weight of fruiting body of strain 26 was the highest (82.01 g), the diameter of cap was the largest (16.00 cm), and the biological efficiency was the highest (23.60%). The content of triterpenoids in fruitus of strain 15 was the highest (1.91%), which was 282.00% higher than that in pharmacopoeia (1.88%). The content of polysaccharides in fruitus ofGanoderma lucidumstrain 15 was the highest (1.27%), which was the highest. The polysaccharide and triterpene contents of isolates 15 and 26 were significantly higher than those of otherGanoderma lucidumstrains. According to the main agronomic traits, biological efficiency and active ingredient contents, Ganoderma Lucidum15 and 26 were suitable for cultivation and promotion in Lhasa area.
参考文献/References:
[1]赵继鼎,张小青.中国真菌志第十八卷:灵芝科[M].北京:科学出版社,2000.[2]BAI J H,XU J,ZHAO J,et al.Ganoderma lucidumpolysaccharide enzymatic hydrolysate suppresses the growth of human colon cancer cells via inducing apoptosis[J].Cell Transplantation,2020,29(1):1-9.[3]徐锦,汪雯翰,杨妍,等.乙醇浓度对提取灵芝三萜含量的影 响及提取物抗前列腺癌细胞LNCaP的活性[J].菌物学报,2020,39(1):155-163.[4]CHIU C H,WANG R,ZHUANG S S,et al.Biotransformation of mogrosides fromSiraitia grosvenoriibyGanoderma lucidum mycelium and the purification of mogroside III E by macroporous resins[J].Journal of Food and Drug Analysis,2020,28(1):74-83.[5]LIU H Z,QIU F F,WANG Y Y,et al.A recombinant protein 〖JP4〗rLZ-8,originally extracted fromGanoderma lucidum,ameliorates OVA-induced lung inflammation by regulating Th17 /Treg balance[J].Journal of Leukocyte Biology,2020,108(2):531-545.[6]田淑雨,鹿士峰,吴杨洋,等.超声破碎辅助提取灵芝多糖工艺优化及抗氧化活性研究[J].食品研究与开发,2019,40(8):101-107.[7]SHIAO M S.Natural products of the medicinal fungusGanoderma lucidum:occurrence,biological activities,and pharmacological functions[J].Chemical Record,2003,3(3):172-180.[8]黄年来,林志彬,陈国良,等.中国食药用菌学:灵芝[M].上海:上海科学技术文献出版社,2010.[9]林志彬.灵芝的现代研究:特性·栽培·成分·药理·应用[M].北京:北京医科大学、中国协和医科大学联合出版社,1996.[10]LI T H,HU H P,DENG W Q,et al.Ganoderma leucocontextum,a new member of theG.lucidumcomplex from southwestern China[J].Mycoscience,2015,56(1):81-85.[11]熊卫萍.西藏白肉灵芝的驯化栽培及其活性成分研究[C]//2016中国南华野生菌大会资料汇编,2016.[12]金鑫,周思菊,李强,等.5种灵芝菌株的农艺性状、活性成分和功效分析[J].四川农业大学学报,2019,37(1):53-59.[13]盛立柱,谢良明,叶晓菊,等.8个灵芝菌株农艺性状、产量及有效成分比较分析[J].中国食用菌,2022,41(1):70-75.[14]李学龙,李跃,张鹏,等.不同灵芝菌株主要农艺性状及活性成分比较分析[J].北方园艺,2022(13):119-125.[15]张彬彬,王震,刘保卫,等.30个野生灵芝菌株遗传多样性及农艺性状评价[J].江苏农业科学,2022,50(2):108-114.[16]齐川,周慧,斯金平,等.不同品种灵芝主要活性成分分析[J].中国实验方剂学杂志,2012,18(17):96-100.[17]付立忠,吴学谦,李明焱,等.灵芝品种子实体多糖和三萜含量分析与评价[J].中国食用菌,2009,28(4):38-40.[18]宋婷婷,张作法,范丽军,等.不同类型灵芝品种的农艺性状、显微结构以及功能成分的比较[J].菌物学报,2020,39(1):34-41.[19]LIU Y J,WHELEN S,HALL B D.Phylogenetic relationships among ascomycetes:evidence from an RNA polymerse II subunit[J].Molecular Biology and Evolution,1999,16(12):1799-1808.[20]马爱瑛.rDNA分析在真菌分类鉴定中的应用[J].安徽农业科学,2010(32):18079,18081.[21]国家药典委员会.《中华人民共和国药典》一 部(2020年 版)[M].北京:中国医药科技出版社,2020.[22]胡惠萍,刘远超,莫伟鹏,等.两株西藏白肉灵芝菌株特性初探[J].食用菌学报,2017,24(1):50-54.[23]莫伟鹏,刘远超,胡惠萍,等.白肉灵芝菌丝体生物学特性的初步研究[J].中国食用菌,2017,36(6):33-38.
相似文献/References:
[1]陈文杰,马清河,古述江.灵芝的椴木栽培技术关键点控制[J].黑龙江农业科学,2012,(12):99.
CHEN Wen-jie,MA Qing-he,GU Shu-jiang.The Key Techniques and Control in Ganoderma Lucidum Basswood Cultivation[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(01):99.
[2]刘 政,闫 晗,王丽威,等.灵芝多糖肽的制备[J].黑龙江农业科学,2010,(10):138.
LIU Zheng,YAN Han,WANG Li-wei,et al.Preparation of Ganoderma lucidum Polysaccharides Peptide[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(01):138.
[3]姜芳燕,马军,陈永敢,等.灵芝活性成分的研究进展[J].黑龙江农业科学,2014,(08):137.
JIANG Fang-yan,MA Jun,CHEN Yong-gan,et al.Research Progress on Active Ingredients of Ganoderma sp.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(01):137.
[4]张现青,邢顺林,宋璇紫,等.拉萨地区葡萄栽培现状分析及对策[J].黑龙江农业科学,2019,(11):132.[doi:10.11942/j.issn1002-2767.2019.11.0132]
ZHANG Xian-qing,XING Shun-lin,SONG Xuan-zi,et al.Analysis on the Current Situation of Grape Cultivation in Lhasa Area and Its Countermeasures[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(01):132.[doi:10.11942/j.issn1002-2767.2019.11.0132]
[5]王赞源,张伟,程虹,等.灵芝连作障碍机制研究[J].黑龙江农业科学,2020,(09):87.[doi:10.11942/j.issn1002-2767.2020.09.0087]
[J].HEILONGJIANG AGRICULTURAL SCIENCES,2020,(01):87.[doi:10.11942/j.issn1002-2767.2020.09.0087]
备注/Memo
收稿日期:2023-08-21