ZHAO Qian.Identification of Potential Pathogenic Genes of Seed Pumpkin Stem Blight Pathogen in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2023,(06):38-44.[doi:10.11942/j.issn1002-2767.2023.06.0038]
黑龙江省籽用南瓜蔓枯菌潜在致病基因挖掘
- Title:
- Identification of Potential Pathogenic Genes of Seed Pumpkin Stem Blight Pathogen in Heilongjiang Province
- 文章编号:
- 8
- 文献标志码:
- A
- 摘要:
- 为了了解籽用南瓜蔓枯病的致病机制，前期鉴定出黑龙江省籽用南瓜蔓枯病的主要病原为Stagonosporopsis cucurbitacearum（Sc.）。在此基础上完成了Sc.1（生长在南瓜叶片上的Sc.菌株）和Sc.2（生长在PDA培养基上的Sc.菌株）组间样品的转录组差异表达分析。共获得319个差异表达基因，其中190个基因下调，129个基因上调；根据GO功能分析，挖掘到58个差异表达基因（DEGs）可能与致病相关；KEGG分析结果显示，挖掘与致病相关的KEGG通路有7个；挖掘到54个编码分泌蛋白基因是差异表达的；与PHI数据库进行比对，共识别到了2 869个基因有较高同源性，其中有96个是差异表达基因；在代谢过程的功能通路中共发现3 327个Sc.基因，结合转录组数据发现86个代谢相关基因差异表达。上述结果表明，真菌寄生在南瓜叶片上的代谢或生物合成途径相关基因的表达量与寄生在PDA培养基上的基因表达量存在着显著差异，这或许是影响Sc.致病性的重要因素。
- Abstract:
- In order to understand the pathogenesis of gummy stem blight about seed pumpkin， Stagonosporopsis cucurbitacearum (Sc.) was identified as the main pathogen of gummy stem blight of seed pumpkin in Heilongjiang Province. In this research， analysis of differential expression transcriptome about Sc.1 (Strain of Sc. growing on pumpkin leaves) and Sc.2 (strain of Sc. growing on PDA medium) were complete. A total of 319 differentially expressed genes were obtained， including 190 down-regulated genes and 129 up-regulated genes. According to GO function analysis， 58 differentially expressed genes were found to be associated with pathogenesis. The results of KEGG analysis showed that there were 7 KEGG pathways associated with pathogenesis. 54 genes encoding secreted proteins were found to be DEGs. Blast with PHI database， 2 869 genes were identified with high homology， among which 96 were DEGs. Total of 3 327 genes were found in the functional pathways of metabolic process， and 86 metabolist-related genes were found to be DEGs. These results indicated that the expression levels of genes related to metabolism or biosynthesis pathway of fungi parasitizing on pumpkin leaves were significantly different from those parasitizing on PDA medium， which may affect Sc. important factors in pathogenicity.
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备注/Memo
收稿日期：2023-01-21