TANG Ke.Comparison of Total Flavonoids Content in Different Parts of Seabuckthorn[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(03):64-67.[doi:10.11942/j.issn1002-2767.2022.03.0064]
沙棘不同部位总黄酮含量比较
- Title:
- Comparison of Total Flavonoids Content in Different Parts of Seabuckthorn
- Keywords:
- seabuckthorn; leaves; fruit; seeds; flavone
- 文献标志码:
- A
- 摘要:
- 为探索沙棘不同亚种间以及相同亚种间不同部位的总黄酮含量差异，本研究以6个不同沙棘品种（系）为试验样品，通过NaNO2-Al(NO3)3-NaOH显色法对不同品种（系）沙棘的叶片、果实及种子中总黄酮含量进行测定。结果表明：所选的6个沙棘品种（系）中叶片黄酮含量为374.37～508.11 mg·（100 g）-1，果实总黄酮含量为78.95～83.78 mg·（100 g）-1，种子总黄酮含量为45.73～69.73 mg·（100 g）-1。不同品种间果实总黄酮含量变化趋势与叶片总黄酮含量变化趋势呈负相关，与种子总黄酮含量变化呈正相关。同为中蒙杂交沙棘亚种的首都杂沙棘与晚黄沙棘各个部位黄酮含量差异悬殊，其中首都杂叶片总黄酮含量最高，为508.11 mg·（100 g）-1，晚黄沙棘果肉和种子中总黄酮含量显著高于其他品种，为83.78和69.73 mg·（100 g）-1。综上所述，参试沙棘品种（系）各部位总黄酮含量平均值均高于对照品种深秋红沙棘，其中不同沙棘品种（系）各部位总黄酮含量存在显著差异（P<0.05），相同沙棘株系叶片中总黄酮平均含量高于果实及种子，杂交沙棘亚种株系各部位总黄酮平均值均略高于蒙古沙棘亚种。
- Abstract:
- In order to explore the differences of total flavonoids content among different subspecies and different parts of the same subspecies of seabuckthorn,six different seabuckthorn varieties (lines) were used as test samples to determine total flavonoids content in leaves,fruits and seeds of different varieties by NaNO2-Al(NO3)3-NaOH chromogenic method.The results showed that the content of total flavonoids in leaves,fruits and seeds of six selected seabuckthorn varieties (lines) were 374.37-508.11,78.95-83.78 and 45.73-69.73 mg·（100 g）-1.The change trend of total flavonoids content in fruits among different varieties(lines) was negatively correlated with that in leaves and positively correlated with that in seeds.The content of total flavonoids in all parts of Shouduza seabuckthorn and Wanhuang seabuckthorn,which are the same subspecies of China Mongolia hybrid seabuckthorn,was very different.Among them,the content of total flavonoids in the leaves of Shouduza seabuckthorn was the highest,whichwas 508.11 mg·（100 g）-1,the content of total flavonoids in the pulp and seeds of Wanhuang seabuckthorn was significantly higher than that of other varieties(lines),which were 83.78 and 69.73 mg·（100 g）-1.To sum up,the average content of total flavonoids in all parts of six seabuckthorn varieties (lines) was higher than that of the control variety Shenqiuhong seabuckthorn,among which there were significant differences in the content of total flavonoids in all parts of different seabuckthorn varieties(lines)(P<0.05).The average content of total flavonoids in leaves of the same seabuckthorn strains was higher than that of fruits and seeds,and the average value of all parts of two hybrid seabuckthorn subspecies was slightly higher than that of Mongolian seabuckthorn subspecies.
参考文献/References:
参考文献： ［1］郑娟,闫益波.沙棘及其副产物在畜牧业中的应用[J].饲料博览,2020(1):56-59. [2]贺静.沙棘酒主发酵过程中不同处理条件下类胡萝卜素、多酚及抗氧化性变化的研究[D].杨凌：西北农林科技大学,2015. [3]赵志侃,苏春香,王峰,等.沙棘全产业链的开发与创新[J].中国食品,2022(1):106-108. [4]崔立柱,付依依,刘士伟，等.沙棘营养价值及产业发展概况[J].食品研究与开发,2021,42(11):218-224. [5]张东,邬国栋.沙棘黄酮的化学成分及药理作用研究进展[J].中国药房,2019,30(9):1292-1296. [6]邢金香.沙棘不同部位总黄酮含量的比较研究[J].山西林业科技,2018,47(3):4-5. [7]施荣富,王春红,李永海,等.沙棘叶黄酮含量及变化规律研究[J].国际沙棘研究与开发,2003(1):40-44. [8]蔡爽,阮成江,杜维,等.沙棘叶片、果肉和种子中黄酮类成分的差异[J].植物资源与环境学报,2019,28(4):58-67. [9]胡建忠,闫晓玲,张东为.3类沙棘叶片主要饲用成分在生长季的变化节律研究[J].现代畜牧兽医,2021(8):19-24. [10]陈春刚,韩芬霞.生物类黄酮的研究与应用综述[J].安徽农业科学，2006(13):2949-2951. [11]闫昌誉,余桂媛,贺晓静,等.沙棘叶的研究进展与产业化应用[J].今日药学,2021,31(7):481-492. [12]李代琼,吴钦孝,张军,等.俄罗斯沙棘优良品种引种试验研究[J].国际沙棘研究与开发,2009,7(1):10-20.
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备注/Memo
收稿日期：2021-12-16 基金项目：黑龙江省农业科学院“农业科技创新跨越工程”专项（HNK2019CX11）。 作者简介：唐克（1984-），男，硕士，助理研究员，从事沙棘育种，栽培繁育技术研究。E-mail：tangke19841102@163.com。