[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(12):22-25.[doi:10.11942/j.issn1002-2767.2019.12.0022]
马铃薯延薯9号茎尖脱毒及成苗分析
- 文献标志码:
- A
- 摘要:
- 马铃薯传统种植方法易引起病毒积累和品种退化，目前国内外利用茎尖脱毒防止马铃薯退化，保证马铃薯高产、稳产已成为一种趋势。本文探究不同消毒方法、不同浓度激素配比的诱导培养基、不同茎尖大小、不同培养条件对茎尖成苗及脱毒对马铃薯品种延薯9号的影响。结果表明：自来水冲洗1~2 min+75%乙醇1 min+0.1%升汞5 min+无菌水冲洗2次，可达到理想的消毒效果；诱导培养基MS+1.0 mg?L-1 IAA+1.0 mg?L-1 GA3+30 g?L-1蔗糖+8 g?L-1琼脂、茎尖分生组织0.3~0.4 mm、光照强度2 000 lx条件下，光照时长16 h?d-1，培养温度26 ℃时成苗效果最好。
参考文献/References:
［1］齐恩芳，王一航，张武，等.马铃薯茎尖脱毒培养方法优化研究[J].中国马铃薯，2007，21（4）：200-202.
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[J].HEILONGJIANG AGRICULTURAL SCIENCES,2020,(12):52.[doi:10.11942/j.issn1002-2767.2020.08.0052]
备注/Memo
收稿日期：2019-06-20