[1]姜宁,章炉军,谭琦,等.香菇菌丝体对四种抗生素的敏感性[J].黑龙江农业科学,2019,(08):119-123.[doi:10.11942/j.issn1002-2767.2019.08.0119]
　JIANG Ning,ZHANG Lu-jun,TAN Qi,et al.Sensitivity of Mycelia of Lentinula edodes Against Four Antibiotics[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(08):119-123.[doi:10.11942/j.issn1002-2767.2019.08.0119]
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香菇菌丝体对四种抗生素的敏感性

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2019年08期 页码: 119-123 栏目: 出版日期: 2019-08-10
Title:
Sensitivity of Mycelia of Lentinula edodes Against Four Antibiotics
作者:
姜宁; 章炉军; 谭琦; 尚晓冬; 董浩然
上海市农业科学院 食用菌研究所，国家食用菌工程技术研究中心，上海 201403
Author(s):
JIANG Ning; ZHANG Lu-jun; TAN Qi; SHANG Xiao-dong; DONG Hao-ran
Institute of Edible Fungi，Shanghai Academy of Agricultural Sciences，National Engineering Research Center of Edible Fungi，Shanghai 201403，China
关键词:
香菇菌丝; 抗生素; 生长速率; 抑制率
Keywords:
mycelium of Lentinula edodes; antibiotics; growth rates; inhibition rates
DOI:
10.11942/j.issn1002-2767.2019.08.0119
文献标志码:
A
摘要:
为探究4种常用抗生素对香菇菌丝体生长的影响，筛选适用于香菇遗传转化的抗生素。本研究以香菇[Lentinula edodes(Berk.)Pegler]沪香F2双核菌丝以及其原生质体单核菌丝为材料，通过测试两者在含有不同种类、不同浓度下抗生素培养皿中的生长速率以及抑制率，探究香菇单双核菌丝对氨苄青霉素、卡那霉素、头孢霉素、潮霉素4种抗生素的敏感性。结果表明：氨苄青霉素、卡那霉素、头孢霉素对香菇单双核菌丝生长以及菌落形态基本没有影响，潮霉素对香菇单双核菌丝生长有强烈的抑制作用，12 mg·L-1的潮霉素能够完全抑制香菇双核菌丝的生长，14 mg·L-1的潮霉素能够完全抑制香菇单核菌丝的生长。卡那霉素不能作为香菇遗传转化的筛选抗生素使用；潮霉素可以用作香菇遗传转化的筛选抗生素；氨苄青霉素和头孢霉素均可以用于对农杆菌的抑制。
Abstract:
In order to investigate the effects of four antibiotics on the mycelium growth of lentinus edodes,screen out suitable antibiotics for genetic transformation of lentinus edodes，we used the mycelium of Lentinula edodes(Berk.)Pegler-huxiang F2 as material,growth rates and inhibition rates were measured in petri dishes containing different types and concentration,the research aimed to investigate that resistance of mycelia of Lentinula edodes against four kind antibiotics,including ampicillin,kanamycin,cefotaxime and hygromycin.The results showed that ampicillin,kanamycin,cefotaximehad no effect on mycelia growth and colony morphology of Lentinula edodes,hygromycin had a strong inhibitory effect on the growth of mushroom mycelium,hygromycin at 12 mg·L-1 could completely inhibit the growth of Lentinula edodes dikaryon mycelia,hygromycin at 14 mg·L-1 could completely inhibit the growth of Lentinula edodes monokaryon mycelia.Kanamycin cannot be used as a screening antibiotic for genetic transformation of Lentinula edodes.Hydramycin can be used as a screening antibiotic for genetic transformation of Lentinula edodes.Ampicillin and cefotaxime could be used to inhibit Agrobacterium.

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备注/Memo

收稿日期：2019-03-23

基金项目：国家现代农业产业技术体系建设专项资金（CARS-20）；上海市食用菌产业技术体系建设专项资金资助（沪农科产字(2019)第9号）。
第一作者简介：姜宁(1991-)，男，硕士，研究实习员，从事食用菌遗传育种研究。E-mail：303526435@qq.com。

更新日期/Last Update: 2019-09-16