ZHANG Yi-ting,LI Ting,YU Bo,et al.Optimization of Extraction Process and Antioxidant Activity of Organic Germanium from Hericium erinaceus[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(12):100-103.[doi:10.11942/j.issn1002-2767.2018.12.0100]
猴头菇有机锗的提取工艺优化及其抗氧化作用
- Title:
- Optimization of Extraction Process and Antioxidant Activity of Organic Germanium from Hericium erinaceus
- 文献标志码:
- A
- 摘要:
- 为进一步开发利用猴头菇，以有机锗提取率为指标，对猴头菇中有机锗提取影响较大的5个因素：NaOH浓度、乙醇浓度、超声时间、浸提时间和浸提温度进行了优化。结果表明：较优提取工艺条件为，NaOH浓度80 g?L-1，乙醇浓度95%，超声时间15 min，浸提时间120 min，浸提温度70 ℃，在此条件下，有机锗提取率为25.34 μg?g-1。在有机锗多糖浓度为2.5 mg?mL-1时，羟自由基（?OH）的清除率达38%，证明其具有较好的体外抗氧化能力。
- Abstract:
- In order to further develop and utilize Hericium erinaceus,the extraction rate of organic germanium was used as the index in this experiment.Five factors affecting the extraction of organic germanium from Hericium erinaceus were optimized,including NaOH concentration,ethanol concentration,ultrasonic time,extraction time and temperature.The results showed that the optimum extraction conditions were NaOH concentration of 80 g?L-1,ethanol concentration of 95%,ultrasound time of 15 min,extraction time of 120 min and extraction temperature of 70 ℃.Under these conditions,the extraction rate of organic germanium was 25.34 μg?g-1.When the concentration of organic germanium polysaccharide was 2.5 mg?mL-1,the scavenging rate of hydroxyl radical (?OH) reached 38%,which proved that it had good antioxidant activity in vitro.
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备注/Memo
收稿日期：2018-07-23