[1]张鑫喆,赵琳琳,徐方旭,等.HPLC不同流动相测定蛹虫草中虫草素和腺苷的比较[J].黑龙江农业科学,2018,(04):130-132.[doi:10.11942/j.issn1002-2767.2018.04.0130]
　ZHANG Xin-zhe,ZHAO Lin-lin,XU Fang-xu,et al.Comparison of Cordycepin and Adenosine in Cordyceps Militaris with Different Mobile Phases by HPLC[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(04):130-132.[doi:10.11942/j.issn1002-2767.2018.04.0130]
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HPLC不同流动相测定蛹虫草中虫草素和腺苷的比较

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2018年04期 页码: 130-132 栏目: 出版日期: 2018-04-10
Title:
Comparison of Cordycepin and Adenosine in Cordyceps Militaris with Different Mobile Phases by HPLC
作者:
张鑫喆1; 赵琳琳1; 徐方旭2; 柳叶飞2; 王升厚2
1.沈阳师范大学 生命科学学院，辽宁 沈阳 110034；2.沈阳师范大学 实验教学中心，辽宁 沈阳 110034
Author(s):
ZHANG Xin-zhe1; ZHAO Lin-lin1; XU Fang-xu2; LIU Ye-fei2; WANG Sheng-hou2
1.College of Life Sciences,Shenyang Normal University,Shenyang 110034,China;2.Experimental Teaching Center of Shenyang Normal University,Shenyang 110034,China
关键词:
高效液相色谱; 流动相; 虫草素; 腺苷
Keywords:
high performance liquid chromatography(HPLC);  mobile phase;  cordycepin;  adenosine
DOI:
10.11942/j.issn1002-2767.2018.04.0130
文献标志码:
A
摘要:
为评价两种不同流动相的可行性，通过测定3种蛹虫草中的虫草素与腺苷的含量，对比高效液相色谱(HPLC)的甲醇-水和乙腈-水两种不同流动相。结果表明：HPLC法的两种不同流动相测定虫草素含量的保留时间分别为6.6和15.0 min；测定腺苷含量的的保留时间分别为5.7 和11.9 min。其中，以甲醇-水为流动相所测得的虫草素含量更高，在检测时更为便捷，保留时间也较适合。
Abstract:
In order to evaluate the feasibility of two different mobile phases,the content of cordycepin and adenosine in 3 species cordyceps militaris was determined by comparing 2 different mobile phases of methanol water and acetonitrile water of high performance liquid chromatography (HPLC).The results showed that the retention time of cordycepin under the 2 different mobile phases in the HPLC method was 6.6 and 15 min,respectively; the retention time for the determination of adenosine content was 5.7 and 11.9 min,respectively.Among them,the content of cordycepin with methanol-water as the mobile phase was higher,and it was more convenient in the detection and the retention time was more suitable.

参考文献/References:

［1］中华人民共和国国家卫生和计划生育委员会.关于批准蛹虫草为新资源食品的公告(2009年第3号)[Z].2015-05-03.
［2］尚红霞,梁晨,易娟.高效液相色谱法同时测定虫草素和腺苷[J].分析科学学报,2011,27(6):776-778.
［3］黄丽俊,李利东,宓晓黎.高效液相色谱法同时检测虫草制品中腺苷和虫草素含量的研究[J].农学学报,2015,5(5):25-28.
［4］NY/T2116-2012.虫草制品中虫草素和腺苷的测定-高效液相色谱法[S].中华人民共和国农业部，2012.
［5］简利茹,杜双田．HPLC与LC-MS/MS测定蛹虫草发酵液中虫草素的方法比较[J].食品科学,2013,34(14)：276-279．

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备注/Memo

收稿日期：2018-01-27第一作者简介：张鑫喆（1992-)，女，在读硕士，从事微生物制药研究。 E-mail：1013932822@qq.com。通讯作者：王升厚(1963-)，男，学士，教授，从事微生物制药研究。E-mail：wshhwq2009@163.com。

更新日期/Last Update: 2018-05-17