[1]王美妮,杨佳利,文斌,等.秋水仙碱诱导狭叶薰衣草多倍体形成条件的研究[J].黑龙江农业科学,2017,(12):21-24.[doi:10.11942/j.issn1002-2767.2017.12.0021]
　WANG Mei-ni,YANG Jia-li,WEN Bin,et al.Study on the Conditions of Polyploid Formation of Lavender Induced by Colchicine[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(12):21-24.[doi:10.11942/j.issn1002-2767.2017.12.0021]
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秋水仙碱诱导狭叶薰衣草多倍体形成条件的研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2017年12期 页码: 21-24 栏目: 出版日期: 2017-12-10
Title:
Study on the Conditions of Polyploid Formation of Lavender Induced by Colchicine
文章编号:
1002-2767（2017）12-0021-04
作者:
王美妮1; 杨佳利1; 文斌1; 谷烨1; 缪天琳2
1．佳木斯大学 农业与环境生物技术研究所，黑龙江 佳木斯 154007; 2.佳木斯大学 生命科学学院，黑龙江 佳木斯 154007
Author(s):
WANG Mei-ni1; YANG Jia-li1; WEN Bin1; GU Ye1; MIU Tian-lin2
1.Agriculture and Environmental Biotechnology Institute of Jiamusi University,Jiamusi,Heilongjiang 154007;2.College of Life Sciences,Jiamusi University,Jiamusi,Heilongjiang 154007
关键词:
薰衣草; 诱变率; 融合率; 多倍体植株
Keywords:
lavender;  mutation rate;  fusion rate;  polyploid plants
分类号:
S573.9;Q813.1+2
DOI:
10.11942/j.issn1002-2767.2017.12.0021
文献标志码:
A
摘要:
为培育性状优良的薰衣草多倍体植株，利用正交法和单因素分析法，进行薰衣草多倍体优良性状筛选试验，探究秋水仙碱诱导染色体加倍的最适浓度和处理时间，聚乙二醇诱导原生质体融合的分子量、工作浓度和诱导时间的最适条件。结果表明：采用秋水仙碱法联合PEG法进行薰衣草多倍体诱导，筛选出具有优良性状的多倍体植株，其中分子量为6 000 Da的PEG在浓度为40%的条件下诱导25 min时，获得的原生质体融合率较高，达到19.6%；薰衣草外植体在浓度为0.2%的秋水仙碱MS诱导培养基中处理48 h诱变频率达60%。运用薰衣草多倍体诱导的试验方法，可快速实现薰衣草多倍体品种的选育，缩短育种周期。
Abstract:
In order to cultivate the exeellent lavender polyploid plant,using orthogonal and single factor,the screening test of high quality characters of lavender polyploidy were carried out,the optimum concentration and treatment time of colchicine to induce chromosome doubling,the optimal conditions of polyethylene glycol-induced protoplast fusion molecular weight,working concentration and induction time were analyzed.The results showed that using colchicine method combined with PEG method for lavender polyploid induction,polyploid plants with excellent traits had been screened out.Among them,the protoplast fusion rate of PEG with a molecular weight of 6 000 Da was 19.6% after being induced at 40% for 25 min.Lavender explants were treated with colchicine MS induction medium at a concentration of 0.20% for 48 h and the mutagenicity frequency reached 60.00%.Therefore,the method of polyploid induction of lavender can quickly realize breeding of polyploid varieties of lavender and shorten the breeding cycle.

参考文献/References:

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备注/Memo

收稿日期：2017-10-27

基金项目:黑龙江省大学生创新创业训练计划资助项目(201710222059)?
第一作者简介:王美妮(1996-),女,黑龙江省绥滨县人,在读学士,从事环境生物学相关研究。E-mail: 664526498@qq.com。
通讯作者:缪天琳(1985-),女,黑龙江省佳木斯市人,硕士,高级实验师,从事生物学相关研究。E-mail: miaotlin@126.com。

更新日期/Last Update: 2018-02-22