[1]林花,车成来,王霞,等.长白山有机黑木耳多糖的提取工艺研究[J].黑龙江农业科学,2017,(10):95-98.[doi:0.11942/j.issn1002-2767.2017.10.0095]
　LIN Hua,CHE Cheng-lai,WANG Xia,et al.Extraction Method of Polysaccharides from Organic Auricularia auricula of Changbai Mountain[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(10):95-98.[doi:0.11942/j.issn1002-2767.2017.10.0095]
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长白山有机黑木耳多糖的提取工艺研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2017年10期 页码: 95-98 栏目: 出版日期: 2017-10-10
Title:
Extraction Method of Polysaccharides from Organic Auricularia auricula of Changbai Mountain
文章编号:
1002-2767（2017）10-0095-04
作者:
林花; 车成来; 王霞; 王欣宇; 王宇辉; 玄东哲; 金龙哲
延边州农业科学院,吉林 延吉 133001
Author(s):
LIN Hua; CHE Cheng-lai; WANG Xia; WANG Xin-yu; WANG Yu-hui; XUAN Dong-zhe; JIN Long-zhe
Yanbian Korean Autonomous Prefecture Academy of Agricultural Sciences,Yanji，Jilin 133001
关键词:
长白山有机黑木耳; 多糖; 提取工艺; 微波辅助提取法
Keywords:
organic Auricularia auricula of Changbai mountain; polysaccharides; extraction technology; microwave assisted extraction
分类号:
S646.6
DOI:
0.11942/j.issn1002-2767.2017.10.0095
文献标志码:
A
摘要:
为了开发微波辅助提取方法，对长白山地区有机黑木耳多糖条件进行优化，确定最佳提取工艺。结果表明：单因子试验和正交试验的最佳条件即以水为提取剂，微波功率为800 W，料液比为1∶40，浸提温度为95 ℃，微波辐射时间为40 min，水浴萃取浸提2次，时间分别为1.5 h，加水量分别为400、500 mL，在此条件下，多糖提取率为19.30%。鞣酸沉淀法脱除蛋白质后，测定黑木耳多糖含量为44.5%。
Abstract:
To explore the extraction methods of microwave assisted extraction,optimizing the conditions on polysaccharides from organic Auricularia auricula of Changbai mountain,finally to determine the best extraction method.The results of single factor test and orthogonal test proved that better extration conditions were water as extraction agent,microwave power 800 W,ratio of material to liquid 1∶40,extraction temperature 95 ℃,the microwave radiation time 40 minutes,water bath extraction time was two times,and the time was 1.5 hours each time,water content were 400 and 500 mL,under these coditions,the extraction rate of polysaccharide was 19.30%.After removing protein by tannic acid method of precipitation,the content of polysaccharides in Auricularia auricula was 44.5%.

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备注/Memo

收稿日期：2017-08-22

基金项目：延边州科技发展计划资助项目（2014AC29）
第一作者简介：林花（1981-），女，吉林省延吉市人，学士，助理研究员，从事天然产物有效成分提取研究。E-mail：664130853@qq.com。
通讯作者：金龙哲（1965-），男，吉林省延吉市人，学士，副研究员，从事食品科学与加工技术研究。E-mail：23124951@qq.com。

更新日期/Last Update: 2018-01-31