[1]盛 慧.蝴蝶兰高效再生体系与遗传转化体系的建立[J].黑龙江农业科学,2017,(04):9-11.[doi:10.11942/j.issn10022767.2017.04.0009]
　SHENG Hui.Establishment of Efficient Regeneration System and Genetic Transformation System of Phalaenopsis[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(04):9-11.[doi:10.11942/j.issn10022767.2017.04.0009]
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蝴蝶兰高效再生体系与遗传转化体系的建立

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2017年04期 页码: 9-11 栏目: 出版日期: 2017-04-10
Title:
Establishment of Efficient Regeneration System and Genetic Transformation System of Phalaenopsis
文章编号:
10022767（2017）04000903
作者:
盛 慧
哈尔滨市农业科学院 生物中心，黑龙江 哈尔滨 150029
Author(s):
SHENG Hui
Biological Center of Harbin Academy of Agricultural Sciences,Harbin,Heilongjiang 150029
关键词:
蝴蝶兰; 再生体系; 遗传转化
Keywords:
Phalaenopsis;  regeneration system;  genetic transformation
分类号:
S682
DOI:
10.11942/j.issn10022767.2017.04.0009
文献标志码:
A
摘要:
为建立蝴蝶兰(Phalaenopsis amabilis)再生体系和遗传转化体系，对蝴蝶兰进行人工授粉，成熟后将胚播种于诱导培养基上诱导原球茎或不定芽。探讨外源调节剂的种类及浓度，并筛选卡那霉素浓度以及除菌剂的种类及浓度。结果表明：当6BA浓度为5 mg?L1，NAA浓度为1 mg?L1时，原球茎的诱导效率最高。当6BA浓度为1.5 mg?L1，同时添加0.5 g?L1活性炭和40 mL?L1椰汁时，壮苗效果最佳。卡那霉素的筛选浓度为4 mg?L1，除菌剂的种类为阿莫西林克拉维酸钾（7∶1），除菌浓度为100 mg?
Abstract:
In order to establish the regeneration system and genetic transformation system of Phalaenopsis amabilis,Phalaenopsis amabilis was used for artificial pollination,after maturation,the embryos were seeded on the induction medium to induce protocorm or adventitious buds. The type and concentration of exogenous regulators were explored,and the concentration of kanamycin and the type and concentration of the fungicide were screened. The results showed that when the concentration of 6BA was 5 mg?L1 and NAA was 1 mg?L1,the induction efficiency of protocorm was the highest. When the concentration of 6BA 1.5 mg?L1,while adding 0.5 g?L1 activated carbon and 40 mL?L1 coconut,the best effect of strong seedlings. The results showed that the concentration of kanamycin was 4 mg?L1,the type of fungicide was amoxicillin clavulanate (7〖DK1〗∶1),and the concentration was 100 mg

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备注/Memo

收稿日期：2017.02.27作者简介：盛慧（1976-），女，辽宁省宽甸县人，博士，高级农艺师，从事植物分子育种研究。Email:shenghui415@aliyun.com。

更新日期/Last Update: 2017-08-06