[1]孙华山,陈 阳,金一锋,等.绿萝叶斑病病原菌鉴定及外源水杨酸对其影响[J].黑龙江农业科学,2017,(03):64-67.[doi:10.11942/j.issn1002-2767.2017.03.0064]
　SUN Hua-shan,CHEN Yang,JIN Yi-feng,et al.Pathogen Identification for Epipremnum aureum Leaf Spot and the Effect of Exogenous Salicylic Acid[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(03):64-67.[doi:10.11942/j.issn1002-2767.2017.03.0064]
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绿萝叶斑病病原菌鉴定及外源水杨酸对其影响

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2017年03期 页码: 64-67 栏目: 出版日期: 2017-03-10
Title:
Pathogen Identification for Epipremnum aureum  Leaf  Spot and the Effect of Exogenous Salicylic Acid
文章编号:
1002-2767（2017）03-0064-04
作者:
孙华山; 陈 阳; 金一锋; 苏 蔚; 李 晨; 王玉书
齐齐哈尔大学 生命科学与农林学院，黑龙江 齐齐哈尔 161001
Author(s):
SUN Hua-shan; CHEN Yang; JIN Yi-feng; SU Wei; LI Chen; WANG Yu-shu
College of Life Science and Agriculture Forestry,Qiqihar University,Qiqihar,Heilonggjiang 161001
关键词:
绿萝; 叶斑病; 水杨酸
Keywords:
Epipremnum aureum;  leaf spot; salicylic acid
分类号:
S682.39
DOI:
10.11942/j.issn1002-2767.2017.03.0064
文献标志码:
A
摘要:
为探明绿萝患病叶片的病原菌，采用形态学鉴定及分子生物学方法鉴定其病原菌来源，进一步探究外源物质对于此病原菌的影响。并采用施加外源水杨酸观测病原菌菌落生长情况、抑菌率及病原孢子萌发情况。结果表明：绿萝病害为叶斑病，此病原菌为枝孢杆菌；在不同浓度外源水杨酸配置的PDA培养基菌落增长速度观察中，低浓度0.1 mmol?L-1SA会促使菌落增长，而随着水杨酸浓度增加，菌落生长速度降低，2.0~10.0 mmol?L-1的测试组始终对病原菌有极高的抑制作用，并没有菌丝体产生。病原孢子数量在不同浓度的水杨酸影响下有着明显的变化。低浓度水杨酸对叶斑病病原孢子生长有促进作用，而0.3、0.5、1.0 mmol?L-1水杨酸对病原菌孢子萌发有着较好的抑制性。
Abstract:
Methods of morphological and molecular biological identification were adopted to ascertain the pathogens of Epipremnum aureum leaf spot.To go a step further on the effect of exogenous factors on pathogens,exogenous salicylic acid was used in the study to observe the growing conditions of pathogen colony,bacteriostatic rate and the germination situation of the pathogen spores.It was found that the Epipremnum aureum had been infected with leaf spot,which fell into Bacillus cladospore.For bacterial colony cultured by PDA medium produced with exogenous salicylic acid of different concentrations,it was noticed that the low concentration of salicylic acid for 0.1 mmol?L-1 would facilitate the growth of bacterial colony.With the growth of salicylic acid concentration,the growth of bacterial colony decelerated.The testing group,experimented with the concentration of 2.0~10.0 mmol?L-1,exerted constantly high inhibiting effects on pathogens,without mycelium generated.With the change in salicylic acid concentration,the number of pathogenic spores varied significantly.Low salicylic acid concentration would facilitate the pathogenic spore growth of leaf spot.However,the salicylic acid concentration for 0.3~1.0 mmol?L-1had a negative effect on the reproduction of pathogenic spores.It exerted a significantly inhibiting effect on the growth of pathogenic spores.

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备注/Memo

收稿日期：2017-02-23
基金项目：国家自然科学基金资助项目（31501785）；黑龙江省大学生创新创业资助项目（201510221019、201610221018）；黑龙江省高等教育学会“十三五”高等教育科研课题资助项目（16Q154）
第一作者简介：孙华山（1993-），男，黑龙江省北安市人，在读学士，从事园林植物逆境生理研究。E-mail：478592528@qq.com。
通讯作者：陈阳（1986-），女，博士，讲师，从事园林植物抗逆分子生物学研究。E-mail：chenyang8368215@126.com。

更新日期/Last Update: 2017-05-04