DENG Kai-yuan,ZHU Yu-he,CHEN Ting,et al.Application of Nano-ZnO in Explant Sterilization of Cirsium setosum[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(08):19-22.[doi:10.11942/j.issn1002-2767.2016.08.0019]
纳米氧化锌消毒小蓟外植体应用研究
- Title:
- Application of Nano-ZnO in Explant Sterilization of Cirsium setosum
- 文章编号:
- 1002-2767(2016)08-0019-04
- Keywords:
- nanometer zinc oxide; Cirsium setosum; tissue culture; sterilization
- 分类号:
- Q94-331;Q7
- 文献标志码:
- A
- 摘要:
- 为使纳米氧化锌在组织培养中达到最佳消毒效果,以小蓟叶片外植体为材料,建立纳米氧化锌消毒的组织培养无菌体系。结果表明:纳米氧化锌浓度为1~7 g?L-1时,对小蓟外植体消毒效果逐渐提升,褐化率也逐渐升高,不同浓度纳米氧化锌对愈伤组织的诱导和生长没有明显的影响。纳米氧化锌最佳消毒处理浓度为5 g?L-1,处理外植体20 min。2 g?L-1纳米氧化锌+0.1% 升汞消毒,外植体大约20%有污染,愈伤组织生长良好。3 g?L-1纳米氧化锌+0.1% 升汞达到最佳的消毒效果,但愈伤组织诱导被抑制,生长缓慢。
- Abstract:
- In order to achieve the best disinfection effect of nanometer zinc oxide (Nano-ZnO) in tissue culture, the explants of thistle leaves as materials, the disinfecting system of Nano-ZnO was established. Thistle explants disinfection effect and browning rate gradually rised, under 1~7 g?L-1 Nano-ZnO. The best disinfection concentration of nano-ZnO was 5 g?L-1 and the best infection time was 20 min. Under 2 g?L-1 Nano-ZnO+0.1% mercuric chloride condition, thistle explants had about 20% pollution and callus grew well. 3 g?L-1 of nano zinc oxide + 0.1% mercuric chloride condition could achieve the best disinfection, but the callus induction was inhibited and grow slowly.
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相似文献/References:
[1]何易航,黄三印,邓凯元,等.不同浓度三种激素对小蓟茎段愈伤组织诱导影响[J].黑龙江农业科学,2015,(09):26.[doi:10.11942/j.issn1002-2767.2015.09.0026]
HE Yi hang,HUANG San-yin,DENG Kai-yuan,et al.Effect of Different Concentrations of Three Kinds of Hormone on ?Callus Induction of ?Cirsium setosum Stem[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(08):26.[doi:10.11942/j.issn1002-2767.2015.09.0026]
备注/Memo
收稿日期:2016-03-10基金项目:华北理工大学在校大学生创新创业训练计划资助项目(X2014059)