ZHOU Fei,HUANG Xu-tang,LIANG Chun-bo,et al.Molecular Cloning and Expression Analysis of Fatty Acid Dehydrogenase Gene FAD2-1 in Oil Sunflower（Helianthus annuus L.）[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(05):8-12.[doi:10.11942/j.issn1002-2767.2016.05.0008]
油用向日葵脂肪酸脱氢酶基因FAD2-1的克隆与表达分析
- Title:
- Molecular Cloning and Expression Analysis of Fatty Acid Dehydrogenase Gene FAD2-1 in Oil Sunflower（Helianthus annuus L.）
- 文章编号:
- 1002-2767（2016）05-0008-05
- Keywords:
- ZHOU Fei; HUANG Xu-tang; LIANG Chun-bo; LI Cen; WANG Wen-jun; MA Jun; LIU Yan
- 分类号:
- S565.5
- 文献标志码:
- A
- 摘要:
- 为了解FAD2-1基因在油用向日葵品质形成过程中的作用，以油用向日葵为材料，利用RT-PCR方法对高含油率保持系材料改HA89（含油率为46%）和低含油率保持系材料86-1（含油率为38%）的脂肪酸脱氢酶基因FAD2-1进行克隆与表达分析。结果表明：获得了脂肪酸脱氢酶基因FAD2-1 全长cDNA编码序列，全长为1 137 bp，编码378个氨基酸。核苷酸序列和蛋白序列比对结果表明，该基因在这2份材料中第44位和第102位核苷酸存在差异，并且都导致了氨基酸的改变。种子发育不同阶段油酸和亚油酸积累动态分析和qRT-PCR研究结果表明,FAD2-1基因表达量变化趋势与亚油酸含量变化趋势基本一致，而与油酸含量变化趋势基本相反。
- Abstract:
- In order to understand the role of FAD2-1 gene in the process of sunflower quality formation,taking oil sunflower as material，full length cDNA coding sequence of the fatty acid dehydrogenase gene in high oil content keep materials Modified HA89(oil content of 46%) and low oil content keep materials 86-1(oil content of 38%)was obtained by RT-PCR method.Sequence analysis showed that the full length of the cDNA was 1 137 bp,encoding 378 amino acids.Nucleotide sequence and protein sequence alignment showed that the 44th and 102nd nucleotides of the gene were different between the two materials,and which both lead to the change of the amino acid.The results of oleic acid and linoleic acid accumulation dynamic analysis in different stages of seed development and qRT-PCR showed that the variation trend of FAD2-1 gene expression was consistent with linoleic acid content,which was opposite to oleic acid content.
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备注/Memo
收稿日期：2016-04-05