TAI Lian-mei,ZUO Yu-hu,ZHANG Ya-ling,et al.Optimization of RAPD-PCR Reaction System on Alternaria solani Causing Potato Early Blight[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(05):4-7.[doi:10.11942/j.issn1002-2767.2016.05.0004]
马铃薯早疫病菌RAPD-PCR反应体系优化
- Title:
- Optimization of RAPD-PCR Reaction System on Alternaria solani Causing Potato Early Blight
- 文章编号:
- 1002-2767（2016）05-0004-04
- 分类号:
- S432.44
- 文献标志码:
- A
- 摘要:
- 为了应用RAPD分子标记技术分析马铃薯早疫病菌遗传多样性，采用单因素水平对影响RAPD反应的Mg2+，dNTPs、TaqDNA聚合酶、引物浓度及引物退火温度等条件进行了优化。结果表明：建立了适宜于早疫病菌的RAPD最佳反应体系，25 μL的反应体系中，Mg2+ 2.5 mmo1·L-1，dNTPs 0.20 mmol·L-1，Taq DNA聚合酶1 U，引物0.40 μmol·L-1。
- Abstract:
- In order to analyze the population genetic diversity of Alternaria solani causing potato early blight by RAPD,single factor design was used to optimize RAPD -PCR reaction on Mg2+，dNTPs,Taq DNA polymerise,primer and annealing temperature.The optimized RAPD system consisted of 2.5 mmo1?L-1 Mg2+，0.20 mmol?L-1 dNTPs,1 U Taq DNA polymerise，0.40 μmol?L-1 primer.
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备注/Memo
收稿日期： 2016-04-04 基金项目：黑龙江省教育厅科学技术研究资助项目（12541587）