[1]薛晓敏,王金政,张安宁,等.三个李品种花粉离体萌发特性与条件筛选试验[J].黑龙江农业科学,2015,(12):11-14.[doi:10.11942/j.issn1002-2767.2015.12.0011]
　XUE Xiao-min,WANG Jin-zheng,ZHANG An-ning,et al.Pollen Germination Characteristics and Conditions Selecting for Three Plum Varieties[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(12):11-14.[doi:10.11942/j.issn1002-2767.2015.12.0011]
点击复制

三个李品种花粉离体萌发特性与条件筛选试验

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2015年12 页码: 11-14 栏目: 出版日期: 2015-12-10
Title:
Pollen Germination Characteristics and Conditions Selecting for Three Plum Varieties
文章编号:
1002-2767（2015）12-0011-04
作者:
薛晓敏; 王金政; 张安宁; 韩雪平
山东省果树研究所，山东 泰安 271000
Author(s):
Pomology Institute of Shandong Province,Tai’an,Shandong 271000?
关键词:
李子; 培养基; 花粉萌发率; 花粉管长度
Keywords:
plum;  culture medium;  pollen germination rate;  pollen tube length
分类号:
S622.3
DOI:
10.11942/j.issn1002-2767.2015.12.0011
文献标志码:
A
摘要:
为了提高李树液体授粉的效率，以3个李品种的花粉为试材，研究了不同培养基和不同培养条件对花粉萌发率和花粉管生长的影响。结果表明：花粉萌发率均随蔗糖浓度、硼酸浓度和培养温度的升高呈现先升后降的抛物线曲线，最佳培养组分为10%蔗糖+100 μL?L-1硼酸，最适培养温度25℃；李花粉萌发较快，培养2 h花粉萌发率达到28%以上，培养4 h花粉萌发率达最高值，此后随培养时间延长花粉萌发率不再增长。在筛好的离体培养条件下测定3个李品种花粉萌发率和花粉管长度，金红李花粉萌发率最高为42.89%，花粉管长度以金艳最高为35.97 μm，早美丽花粉萌发率和花粉管长度在3个品种中最低。
Abstract:
In order to improve the efficiency of liquid pollination in plum,the pollens of 3 plum varieties were used to study the effects of different mediums and different culture conditions on pollen germination rate and pollen tube growth.The results showed that the pollen germination rate was first increased and then decreased with the increase of sucrose concentration,boric acid concentration and incubation temperature,and the optimal medium was 10% sucrose +100 μL?L-1?boric acid,the most suitable culture temperature was 25℃.The plum’s pollen germinated sooner,the germination rate was 28% for 2 h later and reached the maximum value for 4 h later,then the germination rate of pollen was not increased with the time extension.Pollen germination rate and pollen tube length of 3 plum varieties were determined under the selecting condition,pollen germination rate of Jinhong was 42.89% which was the highest among them,and pollen tube length of Jinyan was 35.97 μm which was the longest among them,the pollen germination percentage and pollen tube length of Zaomeili were the lowest among three varieties.

参考文献/References:

[1]刘振岩,李震三.山东果树[M].上海:上海科学技术出版社,2000:715.

[2]任士福,唐秀光,杨建民.李优良品种高效配套技术体系研究与示范推广[J].中国农业科技导报,2005(7):37-41.
[3]薛晓敏,王金政,邹显昌,等.美国李良种优质高产栽培技术总结[J].落叶果树,2008(3):45-47.
[4]王中林.常见果树花粉水悬液配制比例[J].烟台果树,2001（2）:54.
[5]刘海荣.保护地栽植矮甜李授粉方法选择试验[J].中国林副特产,2004（6）:7-8.
[6]吴月亮,赵传统,伊文芝,等.不同培养基及贮藏条件对文冠果花粉生活力的影响[J].北方园艺,2015(5):64-67.
[7]屈海泳,刘连妹,孔文涛,等.桃花粉活性检测和离体萌发特性的研究[J].中国农学通报,2010,26(21):207-212.
[8]李学强,李秀珍,司凤云,等.不同贮藏条件及生长调节剂对欧李花粉生活力的影响[J].西北植物学报,2007,
27(11):22512256.
[9]宋利霞.培养基种类对果树花粉发芽率的影响[J].河北果树,2011(5):1011.
[10]赵长星,刘成连.培养基种类及蔗糖浓度对部分果树花粉发芽率的影响[J].河北林果研究,2001,16(3):240-243.
[11]周怀军,赵阿曼,刘从霞,等.李杏花粉生活力试验研究[J].河北林业科技,2001(3):5-9.
[12]田寿乐,孙晓莉,沈广宁,等.中国栗属植物花粉萌发特性研究[J].经济林研究,2013,31(5):75-80.
[13]韩雪平,薛晓敏,王金政,等.‘凯特’杏花粉离体培养条件筛选[J].北方园艺，2015(3):96-99.
[14]李桂云,顾景梅,王峰.不同培养基对果树花粉发芽率影响的试验[J].山西果树,2001,2(1):4-5.
[15]李玉萍,罗凤霞,王春彦,等.风信子花粉萌发及贮藏性研究[J].西北植物学报,2010,30(12): 2484-2489.
[16]覃守俊,张红艳,陈安凤,等.不同李品种花粉生活力观察[J].湖北林业科技,1999(2):18-19.
[17]李颖岳,续九如,史良,等.台湾青枣不同品种花粉萌发和生活力测定[J].果树学报,2005,22(6):728-730.

相似文献/References:

[1]尹相博,于咏梅,于立芝.红树莓组织培养研究进展[J].黑龙江农业科学,2013,(02):140.
　YIN Xiang-bo,YU Yong-mei,YU Li-zhi.Review of Progress in Tissue Culture of Red Raspberry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(12):140.
[2]武佩祥,邱彩玲,魏 琪,等.脱毒马铃薯试管薯简化生产技术的研究[J].黑龙江农业科学,2012,(06):37.
　WU Pei-xiang,QIU Cai-ling,WEI Qi,et al.Research on Simplify Production Technologyfor Virus-free Seed Potato Microtubers[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(12):37.
[3]王禹.树莓初代培养研究[J].黑龙江农业科学,2012,(05):85.
　WANG Yu.Study on Initial Culture of Raspberry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(12):85.
[4]刘晓晨,曹君迈,陈彦云,等.罗布麻外植体的筛选及愈伤组织诱导[J].黑龙江农业科学,2012,(09):24.
　LIU Xiao-chen,CAO Jun-mai,CHEN Yan-yun,et al.Explants Selection and Callus Induction of Apocynum venetum L.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(12):24.
[5]邱广伟,夏平,夏静波,等.紫色马铃薯茎尖培养基筛选试验[J].黑龙江农业科学,2011,(03):24.
　QIU Guang-wei,XIA Ping,XIA Jing-bo,et al.Selection of Stem Culture Medium of Purple Potato[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(12):24.
[6]刘建军,周金梅.草莓花药培养中诱导不定芽情况研究[J].黑龙江农业科学,2011,(11):13.
　LIU Jian-jun,ZHOU Jin-mei.Study on the Inducing Adventitious Buds inAnther Culture of Fragaria×ananassa in vitro[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(12):13.
[7]盖玉红,魏 健.不同培养基条件对红景天花药愈伤组织生长量的影响[J].黑龙江农业科学,2015,(04):10.[doi:10.11942/j.issn1002-2767.2015.04.0010]
　GAI Yu-hong,WEI Jian.Effect of Different Culture Medium on the Plant Tissue Growth of Rhodiola sachalinensis Anther Callus[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(12):10.[doi:10.11942/j.issn1002-2767.2015.04.0010]
[8]汪淑芳,梁 梓,杨瑶君,等.绵竹快繁灭菌及丛芽诱导研究[J].黑龙江农业科学,2015,(07):19.[doi:DOI:10.11942/j.issn1002-2767.2015.07.0019]
　WANG Shu-fang,LIANG Zi,YANG Yao-jun,et al.Study on Disinfection Method and Multiple Shoot Induction of Bambusa intermedia ?Hsueh et Yi[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(12):19.[doi:DOI:10.11942/j.issn1002-2767.2015.07.0019]
[9]陈兆贵,殷　芳,钟志铭.马铃薯微型薯诱导因素的研究[J].黑龙江农业科学,2009,(05):10.[doi:10.11942/j.isn1002-2767.2009.05.0010]
　CHEN Zhao-gui,YIN Fang,ZHONGZhi-ming.Study on Influence Factors of Potato Micro-tuber Inducement[J].HEILONGJIANG AGRICULTURAL SCIENCES,2009,(12):10.[doi:10.11942/j.isn1002-2767.2009.05.0010]
[10]李大林,张云江,刘大力.水稻花药培养方法改良探讨[J].黑龙江农业科学,2000,(02):19.[doi:10.11942/j.issn1002-2767.2000.02.0019]
　Li Dalin,Zhang Yunjiang.Discussion on Improvement of Method of Rice Anther Culture[J].HEILONGJIANG AGRICULTURAL SCIENCES,2000,(12):19.[doi:10.11942/j.issn1002-2767.2000.02.0019]

备注/Memo

收稿日期：2015-09-21

第一作者简介：薛晓敏（1979-），女，河北省邯郸县人，硕士，助理研究员，从事水果遗传育种与栽培研究。E-mail：xuexiaomin79@126.com。

更新日期/Last Update: 2016-11-22