[1]南芝润,惠国强,罗 ?绮,等.超声波辅助花粉介导法获得转BtCry1Ac基因玉米的研究[J].黑龙江农业科学,2015,(09):22-25.[doi:10.11942/j.issn1002-2767.2015.09.0022]
　NAN Zhi-run,HUI Guo-qiang,LUO Qi,et al.Research on ?BtCry1Ac ?Transgenic Maize Lines Using ?Ultrasonic assisted Pollen Mediated Plant Transformation Method[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(09):22-25.[doi:10.11942/j.issn1002-2767.2015.09.0022]
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超声波辅助花粉介导法获得转BtCry1Ac基因玉米的研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2015年09 页码: 22-25 栏目: 出版日期: 2015-09-10
Title:
Research on ?BtCry1Ac ?Transgenic Maize Lines Using ?Ultrasonic assisted Pollen Mediated Plant Transformation Method
文章编号:
1002-2767（2015）09-0022-04
作者:
南芝润1; 惠国强1; 罗 ?绮1; 宋红霞4; 吴家和2; 孙 毅3; 张红梅1
1.山西省农业科学院 玉米研究所，山西 忻州 034000；2.中国科学院 微生物研究所，北京 100101；3.山西省农业科学院 生物技术研究中心，山西 太原 030031；4.山西大学 生物工程学院，山西 太原 030031
Author(s):
NAN Zhi-run1; HUI Guo-qiang1; LUO Qi1; SONG Hong-xia4; WU Jia-he2; SUN Yi3; ZHANG Hong-mei1
1.Maize Research Institute of Shanxi Academy of Agricultural Sciences,Xinzhou，Shanxi 034000;2.Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101; 3.Biotechnology Research Centerof ?Shanxi Academy of Agricultural Sciences,Taiyuan，Shanxi 030031；4.Biological Engineering College of Shanxi University，Taiyuan，Shanxi 030031
关键词:
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Keywords:
maize(Zea may L.);  Cry1Ac ?gene;  ultrosonicassisted pollen mediated plant transformation method;  resistance to insect
分类号:
S513
DOI:
10.11942/j.issn1002-2767.2015.09.0022
文献标志码:
A
摘要:
为了进一步分析玉米基因功能及基因转化，并为玉米抗虫提供种质资源，利用超声波辅助花粉介导法，将抗虫基因Cry1Ac?导入玉米优良自交系昌7-2中，获得大量的转基因植株。结果表明：转基因植株经过草铵膦抗性筛选、PCR检测、BT蛋白试纸条检测和室内抗虫性鉴定等分析，最后获得高抗玉米螟的转?BtCry1Ac?基因株系9个。培育出了高抗玉米螟的种质资源材料，同时也建立了超声波辅助花粉介导转化玉米的高效转化体系，该方法是简捷、快速有效、无基因型限制的植物转化方法。
Abstract:
In order to further analyze the gene functions and transformation of maize,and provide germplasm resources for maize insectresistant. The insect resistant ?Cry1Ac??gene was used to transfer into maize inbred lines Chang 7-2,through the method of ultrasonicassisted pollen mediated transformation,more transgenicmaize plantlets were obtained.The results showed that the transgenic plants were detected by PCR detection,Bt protein strip and raising insect assay in laboratory.Nine elite transgenic lines with high resistance to Ostrinia nubilalis were developed.In conclusion,it not only provided germplasm resources for maize beeding but also established the ultrasonic processing pollen mediated maize genetic transformation method,which was a simple,rapid and effective method for the transformation of plants.

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备注/Memo

收稿日期：2015-05-08

基金项目：国家农业部转基因专项资助项目（2008ZX08003-001）
第一作者简介：南芝润（1979-），女，山西省运城市人，硕士，助理研究员，从事玉米转基因育种研究。E-mail:yexue139@126.com。

更新日期/Last Update: 2016-04-01