GAI Yu-hong,WEI Jian.Effect of Different Culture Medium on the Plant Tissue Growth of Rhodiola sachalinensis Anther Callus[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(04):10-12,37.[doi:10.11942/j.issn1002-2767.2015.04.0010]
不同培养基条件对红景天花药愈伤组织生长量的影响
- Title:
- Effect of Different Culture Medium on the Plant Tissue Growth of Rhodiola sachalinensis Anther Callus
- 文章编号:
- 1002-2767(2015)04-0010-03
- Keywords:
- Rhodiola sachalinensis anther callus; growth; medium; auxin; cytokinins
- 分类号:
- S567
- 文献标志码:
- A
- 摘要:
- 为进一步开发利用红景天资源,以红景天花药诱导的愈伤组织为材料,研究了不同培养基、不同种类和浓度的生长素和细胞分裂素对花药愈伤组织生长量的影响。结果表明:培养基MS和NB对花药愈伤组织生长量的影响无显著差异;低浓度的生长素对红景天花药愈伤组织生长量有利,2,4-D对愈伤组织生长的影响略高于NAA。高浓度的6BA利于在景天花药愈伤组织生长,在含有相同浓度NAA的培养基中,6-BA比KT适合花药愈伤组织生长。
- Abstract:
- In order to further develop and utilize the resource of Rhodiola sachalinensis,taking its callus induction anther as materials,the effect of different medium,different type and concentration of plant hormone and cytokin on anther callus growth were studied.The results showed that on anther callus growth had no significant difference,the lower concentration of IAA could promote the grouth of anther callus,and the effect of 2,4-D was higher than that of NAA.The higher concentration of 6-BA could promote the qrowth of anther callus.With the same basal medium,6BA was more suitable for the growth of anther callus than KT.
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备注/Memo
收稿日期:2014-10-21
第一作者简介:盖玉红(1980-),女,吉林省东丰县人,博士,实验师,从事作物遗传育种研究。E-mail:gyh0214@qq.com。
通讯作者:魏健(1980-),男,博士,讲师,从事细胞遗传学研究。