[1]乐薇,吴士筠.AlCl3比色法测定箬叶总黄酮含量[J].黑龙江农业科学,2015,(02):98-101.[doi:10.11942/j.issn1002-2767.2015.02.0098]
　YUE Wei,WU Shi-yun.Determination of Total Flavonoids from Indocalamus Leaves by AlCl3 Colorimetric Method[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(02):98-101.[doi:10.11942/j.issn1002-2767.2015.02.0098]
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AlCl3比色法测定箬叶总黄酮含量

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2015年02 页码: 98-101 栏目: 出版日期: 2015-02-10
Title:
Determination of Total Flavonoids from Indocalamus Leaves by AlCl3 Colorimetric Method
文章编号:
1002-2767（2015）02-0098-04
作者:
乐薇; 吴士筠
武汉工商学院 环境与生物工程学院
Author(s):
YUE Wei; WU Shi-yun
College of Environmental and Biological Engineering
关键词:
箬叶; 总黄酮; AlCl3;  比色法
Keywords:
indocalamus leaves;  total flavonoids;  AlCl3;  colorimetric method
分类号:
R284
DOI:
10.11942/j.issn1002-2767.2015.02.0098
文献标志码:
A
Abstract:
In order to study the simple and standard method for measuring indocalamus leaves,taking indocalamus leaves as material,A AlCl3 colorimetric method for the determination of total flavonoids from indocalamus leaves was established,the effect of solvent,the dosage of AlCl3,pH and standing time were studied.The results showed that the optimum conditions were 50% ethanol as solvent,added 0.025 mol?L-1 AlCl3 with pH 5~6,after 20 min total flavonoids reacts with AlCl3 to form a stable yellow complex,the determine wavelength was 415 nm.The regression equation was A=0.024 6c+0.018 1，correlation coefficient R2  was 0.999 2.The recovery was 97.94%~103.8%,and RSD was 2.4%.

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备注/Memo

收稿日期：2014-10-10
基金项目：湖北省自然科学基金资助项目（2013C111）
第一作者简介：乐薇（1979-），女，湖北省沙市人，硕士，副教授，从事生物分析方面研究。E-mail：yuewei11@126.com。

更新日期/Last Update: 2015-05-26