LI Shu-mei,WANG Fu-juan,DONG Li-ping,et al.Change of Seed Vigor and Physiological and Biochemical Characteristics of Barley with Artificial Aging Treatment[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(01):133-136.[doi:10.11942/j.issn1002-2767.2015.01.0133]
黑麦草老化处理后种子活力和生理生化特性的变化
- Title:
- Change of Seed Vigor and Physiological and Biochemical Characteristics of Barley with Artificial Aging Treatment
- 文章编号:
- 10022767（2015）01013303
- Keywords:
- perennial ryegrass; artificial aging; germination index; physiological and biochemical indicators
- 分类号:
- S563.2
- 文献标志码:
- A
- 摘要:
- 为延长黑麦草的种子寿命及其安全贮藏，以黑麦草邦德为试验材料，采用45℃，90%相对温度的条件进行人工老化处理，并对其种子发芽指标、相对电导率、丙二醛含量、可溶性糖、可溶性蛋白、POD和CAT活性等生理生化指标进行了测定。结果表明：相对电导率、可溶性糖和丙二醛含量等随着老化时间的延长逐渐升高，可溶性蛋白、POD和CAT活性等逐渐降低，表明黑麦草种子耐贮藏的适宜老化时间为3 d。通过研究可知，在大麦种子的老化过程中，相对电导率、可溶性糖含量、可溶性蛋白质含量、POD和CAT活性均与种子的老化及劣变密切相关，可据此检测种子活力。
- Abstract:
- In order to prolong the life of perennial ryegrass seeds and safe storage,with ryegrass Bond as experimental material,using 45℃,90% RH conditions of artificial aging treatment,and the seed germination index,relative conductivity,MDA content,soluble sugar,soluble protein,POD and CAT activity and so on physiological and biochemical indexes were determined.The results showed that the relative electrical conductivity,soluble sugar and MDA content increased with aging time prolong,such as soluble protein,POD and CAT activity gradually reduced,suggests that the suitable aging time of perennial ryegrass seed storage tolerance for 3 d.Through research showed that in the process of barley seed aging,relative electrical conductivity,soluble sugar content,soluble protein content,POD and CAT activities were closely related with seed aging and deterioration,shall be tested on the basis of seed vigor.
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备注/Memo
收稿日期：2014-08-25
基金项目：信阳农林学院青年教师科研基金资助项目（2012-01009）
第一作者简介：李淑梅（1979-），女，河北省衡水市人，硕士，讲师，从事种子检验及作物遗传育种方面的教学和科研工作。E-mail：lishumei79@163.com。