LIU Wen-ping,LIU Jian-xin,NAN Xiang-ri,et al.Culture and Plantlets Regeneration from Protoplast of Diploid Potato[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(01):6-9.[doi:10.11942/j.issn1002-2767.2015.01.0006]
二倍体马铃薯原生质体培养与植株再生
- Title:
- Culture and Plantlets Regeneration from Protoplast of Diploid Potato
- 文章编号:
- 1002-2767（2015）01-0006-04
- Keywords:
- diploid potato; protoplast; regeneration plantlets
- 分类号:
- S532
- 文献标志码:
- A
- 摘要:
- 原生质体培养是体细胞杂交的基础，同时也是获得变异材料的重要手段。以引进的4个二倍体马铃薯为材料，研究了渗透浓度、酶液组成、酶解时间及材料预处理对原生质体产量和活力的影响。结果表明：预处理是原生质体成活、分裂及后期发育的重要条件，处理D预处理效果最好，原生质产量和活力最高，且有较多细胞分裂；马铃薯原生质体适宜的渗透浓度为0.35～0.40 mol.L-1；RH2和RH3酶解时间以4 h为宜，RH1和RH4酶解时间以4.5 h为宜；酶解液为2.0%纤维素酶和0.2%果胶酶的混合液。基因型对原生质体培养影响较大。经过培养，有2个二倍体材料获得了再生植株。
- Abstract:
- Protoplast culture is the foundation of somatic hybridization and an important method to acquire mutant material.Taking four introduced diploid potato as materials,the effect of osmotic concentration,enzymolysis conduction，enzymolysis time and pretreatment on yield and vitality of protoplast were studied.The results indicated that pretreatment was a key factor for surviving,division and development of protoplast.The protoplast yield and vitality of treatment D were the best.The osmotic concentration suitable for protoplast was 0.35～0.40 m·L-1 .The suitable enzymolysis time for RH2 and RH3 was 4 h,RH1 and RH4 was 4.5 h.Enzymatic hydrolysate was 2.0% Cellulase RS Onozuka and 0.2% Pectolyase Y-23.The protoplast culture was greatly influenced by genotypes.Regeneration plantlets of two diploid potato materials were successfully gained.?
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备注/Memo
收稿日期：2014-07-29
基金项目：科技部国家国际科技合作资助项目（2011-DFR30840）
第一作者简介：刘文萍（1963-），女，黑龙江省哈尔滨市人，学士，研究员，从事生物技术研究。E-mail：liuwenping63@163.com。