[1]张天静,雷家军,孙文松.菊花品种神马的茎段离体快繁研究[J].黑龙江农业科学,2014,(12):19-22.
　ZHANG Tian-jing,LEI Jia-jun,SUN Wen-song.Study on Stem Micropropogation of Chrysanthemum cv.Jinba[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(12):19-22.
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菊花品种神马的茎段离体快繁研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2014年12 页码: 19-22 栏目: 出版日期: 2014-12-10
Title:
Study on Stem Micropropogation of Chrysanthemum cv.Jinba
文章编号:
1002-2767（2014）12-0019-04
作者:
张天静1; 雷家军2; 孙文松1
1.辽宁省经济作物研究所，辽宁 辽阳 111000；
2.沈阳农业大学 园艺学院，辽宁 沈阳 110866
Author(s):
ZHANG Tian-jing1; LEI Jia-jun2; SUN Wen-song1
1.Economic Crop Institution of Liaoning Province,Liaoyang,Liaoning 111000；
2.College of Horticulture,Shenyang Agricultural University,Shenyang,Liaoning 110866
关键词:
菊花; 茎段; 离体快繁
Keywords:
Chrysanthemumcv.Jinba; stem; rapid propogation in vitro
分类号:
S682.1
文献标志码:
A
摘要:
为加快优质菊花种苗的繁殖，以菊花品种神马的茎段为试材，建立了无菌操作体系，并进行继代增殖、生根培养和驯化移栽。结果表明：升汞消毒茎段5 min效果最佳，存活率可达70.0%，污染率为13.30%；最佳初代培养基为MS+BA 1.0 mg·L-1+NAA 0.1 mg·L-1，萌发率高且生长良好；最佳增殖培养基为MS+BA1.0 mg·L-1+NAA0.1 mg·L-1，增殖系数达到6.2；最佳生根培养基为1/2MS+NAA0.1 mg·L-1，生根率可达97.8%。
Abstract:
In order to speed up the breeding of high-quality Chrysanthemum cv.Jinba,taking stem segment of Chrysanthemum cultivar Jinba materials,sterile system was established,and the axillary bud in vitro was subcultured,rooted,domesticated and transplanted.The results showed that the disinfection time by 0.1% HgCl2 for 5 minutes was optimal,and the survival rate could be up to 70.0%,and contamination rate was 13.3%.When the induction medium was MS+BA 1.0 mg·L-1+NAA 0.1 mg·L-1,the germination rate was high and the plantlets grew well.The optimal subculture medium was MS+BA1.0 mg·L-1+NAA0.1 mg·L-1 with multiplication coefficient of 6.2,and the most suitable rooting medium was 1/2MS+NAA0.1 mg·L-1,with rooting rate over 97.8%.

参考文献/References:

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[2]李启任,彭恒,王东.孔雀菊组织培养快速繁殖研究[J].云南大学学报：自然科学版,1997,19(4):370-373.

[3]田郎,谭海燕.菊花试管苗的研制及其规模化生产[J].福建热作科技,1999,24(1):1-3.

[4]孙月剑,车冬梅.欧洲矮化大花萱草组织培养的研究[J].大连民族学院学报,2006,8(3):44-46.

[5]向太和.菊花组织培养植株再生及其后代的变异[J].杭州师范学院学报：自然科学版,2006,5(l):42-45.

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[7]Sudhersan C,Aboel-Nil M,Hussain J.Tissue culture technology for the conservation and propagation of certain native plants[J].Journal of Arid Environments,2003,54(1):133-147.


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备注/Memo

基金项目：沈阳市农业科技共建资助项目(201423)

第一作者简介：张天静（1979-），女，辽宁省辽阳市人，在读硕士，助理研究员，从事植物组织培养研究。E-mail：zhangtianjing0903@163.com。
通讯作者：雷家军（1966-），男，辽宁省沈阳市人，博士导师，教授，从事观赏植物科研教学工作。E-mail：jiajunleisy@163.com。

更新日期/Last Update: 2014-12-24