[1]张丽敏,邓晨光,齐兴田,等.苹果过氧化物酶活性及同工酶分析[J].黑龙江农业科学,2014,(11):18-20.
　ZHANG Li-min,DENG Chen-guang,QI Xing-tian,et al.Analysis on POD Activity and Isozyme of Apple[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(11):18-20.
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苹果过氧化物酶活性及同工酶分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2014年11 页码: 18-20 栏目: 出版日期: 2014-11-10
Title:
Analysis on POD Activity and Isozyme of Apple
文章编号:
1002-2767（2014）11-0018-03
作者:
张丽敏1; 邓晨光1; 齐兴田2; 程海涛1; 张宇3
1.佳木斯大学 生命科学学院，黑龙江 佳木斯 154007；2.佳木斯大学 理学院，黑龙江 佳木斯 154007；3.佳木斯大学 药学院，黑龙江 佳木斯 154007
Author(s):
ZHANG Li-min1; DENG Chen-guang1; QI Xing-tian2; CHENG Hai-tao1; ZHANG Yu3
1.College of Life Science,Jiamusi University,Jiamusi,Heilongjiang 154077;2.College of Science,Jiamusi University,Jiamusi,Heilongjiang 154077;3.College of Pharmacy,Jiamusi University,Jiamusi,Heilongjiang 154077
关键词:
苹果; 过氧化物酶; 同工酶; 聚丙烯酰胺凝胶电泳; 愈创木酚比色法
Keywords:
apple; POD; isozyme; PAGE; guaiacol colorimetric method
分类号:
S661.1
文献标志码:
A
摘要:
为了给苹果生产中选种、育种和栽培提供生化依据，利用聚丙烯酰胺垂直板凝胶电泳技术对7种苹果的过氧化物酶同工酶进行分析，并采用愈创木酚法测定过氧化物酶活性。结果表明：不同品种苹果的过氧化物酶同工酶有一定差异，愈创木酚法测得的苹果过氧化物酶活性与过氧化物酶同工酶谱的表达活性基本一致；乔纳金和黄元帅POD同工酶谱型很相近，亲缘性相近；奶油富士、烟台富士、冰糖果和国光POD同工酶谱相近，遗传上接近。
Abstract:
In order to provide biochemical basis for seed selection,breeding and cultivation in apple production,peroxidase isozyme of apple was analyzed by PAGE and peroxidase activity was measured.The results showed that there were some differences of peroxidease isozyme in different apple varieties.The peroxidase activity measured by guaiacol colorimetric method were consistent with peroxidase isozyme patterns of apple;the POD isozyme patterns of Jonagold was similar to Golden Delicious,their affinity was similar;POD isozyme patterns of Cream Fuji apple,Yantai Fuji apple,Bingtangguo and Ralls apple were similar and their genetic characteristics were similar.

参考文献/References:

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备注/Memo

收稿日期：2014-07-10基金项目：黑龙江省卫生厅科研项目（2011-454）第一作者简介：张丽敏（1972-），女，黑龙江省佳木斯市人，硕士，副教授，从事遗传学及植物同工酶研究。E-mail:13104549106@163.com。

更新日期/Last Update: 2014-12-12