[1]李烨,金丹丹,谢立波,等.茄子高频再生体系的建立与优化[J].黑龙江农业科学,2014,(09):48-52.
　LI Ye,JIN Dan-dan,XIE Li-bo,et al.Establishment and Optimization of High-frequency Regeneration System of Eggplant[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(09):48-52.
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茄子高频再生体系的建立与优化

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2014年09 页码: 48-52 栏目: 出版日期: 2014-09-10
Title:
Establishment and Optimization of High-frequency Regeneration System of Eggplant
文章编号:
1002-2767（2014）09-0048-05
作者:
李烨1; 金丹丹2; 谢立波3; 李景富2
1.哈尔滨市农业科学院，黑龙江 哈尔滨 150001；
2.东北农业大学，黑龙江 哈尔滨 150030；
3.黑龙江省农业科学院，黑龙江 哈尔滨 150086
Author(s):
LI Ye1; JIN Dan-dan2; XIE Li-bo3; LI Jing-fu2
1.Harbin Academy of Agriculture Sciences,Harbin,Heilongjiang 150001；
2.Northeast Agriculture University，Harbin,Heilongjiang 150030；
3.Heilongjiang Academy of Agriculture Sciences,Harbin,Heilongjiang 150023
关键词:
茄子; 外植体; 再生
Keywords:
eggplant; explants; regeneration
分类号:
S641.1
文献标志码:
A
摘要:
为建立并优化茄子高频再生体系，研究以茄子品种哈农杂茄2号和哈农杂茄3号为材料，利用不同的外植体和植物生长调节剂组合，探讨影响茄子再生频率的主要因素。结果表明：茄子最佳再生子叶最适分化培养基为MS 0+6-BA 1 mg.L-1+KT 2 mg.L-1；下胚轴最适分化培养基为MS 0+KT 2 mg.L-1，再生苗最佳生根培养基为1/2MS+IAA 0.1 mg.L-1，且下胚轴的分化效率高于子叶。
Abstract:
In order to establish and optimize the high-frequency regeneration system of eggplant,taking cotyledonary petiole and hypocotyl explants of Hanongzaqie 2 and Hanongzaqie 3 as materials,the shoot regeneration frequencies of two eggplant(Solanum melongena L)cultivars were examined to discuss the factors affecting regeneration rate.The results showed that the optimum shoot inducing mediums for cotyledons was MS 0+6-BA 1 mg.L-1+KT 2 mg.L-1;the optimum shoot inducing mediums for hypocotyls was MS 0+KT 2 mg.L-1,the best rooting medium for regeneration seedling was 1/2MS+IAA 0.1 mg.L-1.And the differentiation of hypocotyls was higher than that of cotyledons.

参考文献/References:

[1]程继鸿,赵福宽.弱光条件下茄子花药培养与再生体系的建立[J].北京农学院学报.2000,16(2):22-26.
[2]张英兰,李耿光.两种茄子子叶诱导胚状体和植株再生[J].植物生理学通讯，1987（4）:56.
[3]Gleddie S,Keller W,Setterfield G.Somatic embryogenesis and plant regeneration from leaf explants and cell suspensions of Solanum melongena(eggplant)[J].Canadian Journal of Botany，1983，61:656-666
[4]Kamat MG,Rao PS.Vegetative multiplication of eggpl-ants(Solanum melongena)using tissue culture techni-ques[J].Plant Science Letters，1978，13:57-65.
[5]许勇,王福钧,周长久.粘毛茄子紫叶原生质体的培养及植株再生[J].植物生理学报,1990(4):27-29.
[6]Alicchio R,Antonioli C,Palenzona D.Karyotypic variability in plants of Solanum melongena regenerated from callus grown in presence of culture filtrate of Verticillium dahlia[J].Theoretical and Applied Genetics，1984,67:267-271.
[7]Matsuoka H,Hinata K.NAA-Induced Organogenesis and Embryogenesis in Hypocotyl Callus of Solarium melongena L[J].Journal of Experimental Botany,1979,30:363-370.
[8]颜敬昌.植物组织培养手册[M].上海:上海科学技术出版社，1989：289-292.
[9]Saxena PK,Gill R,Rashid A.Plantlet formation from isolated protoplasts of Solanum melongena L[J].Protoplasma,1981,106:355-359.
[10]余波澜,张利明,孙勇如,等.茄子子叶和下胚轴的组织培养和植株再生[J].植物生理学通讯，2003，39(4):317-320.
[11]黎定军,张宝玺,赵开军,等.辣椒子叶高效植株再生体系的建立[J].园艺学报,2002，9（1）：25-29.
[12]邹克勤,张银东.几丁质酶和抗菌肽D双价基因转化茄子的研究[J].热带作物学报,2001,34(2):57-61.

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备注/Memo

收稿日期：2014-04-16
第一作者简介：李烨（1972-），女，黑龙江省绥化市人，硕士，研究员，从事蔬菜作物遗传育种研究。E-mail：eggplant_2010@163.com。
通讯作者：李景富（1942-），男，黑龙江省绥化市人，学士，教授，从事蔬菜作物遗传育种研究。E-mail:Lijf_2005@126.com。

更新日期/Last Update: 2014-12-04