LI Yu-zhong,WANG Tong,WANG Bin,et al.Separation Identification and Effects Test of Leaf Spot Pathogen of Ligustrum vicaryi[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(08):62-64.
金叶女贞叶斑病菌的分离鉴定与药效试验
- Title:
- Separation Identification and Effects Test of Leaf Spot Pathogen of Ligustrum vicaryi
- 文章编号:
- 1002-2767(2014)08-0062-03
- Keywords:
- Ligustrum vicaryi; pathogen; identification; fungicide effect
- 分类号:
- S436.8
- 文献标志码:
- A
- 摘要:
- 为了鉴定金叶女贞叶斑病的致病菌,寻找有效的防治药剂,通过组织分离法和形态学方法对引起金叶女贞叶斑病的病原菌进行了分离、纯化和鉴定,采用平皿菌丝生长抑制法测定了3种杀菌剂对该病原菌的抑制效果。结果表明:引起衡阳地区金叶女贞叶斑病的病原菌为蔓荆子棒孢(Corynespora viticis),50%多菌灵和75%百菌清对该病菌的生长有较好的抑制效果,EC50分别为1.782和2.080 g.L-1,可作为该病的有效防治药剂。
- Abstract:
- In order to identify leaf spot pathogenic of Ligustrum vicaryi and find out effective pesticide to control it,leaf spot pathogen was isolated,purified and identified using organizing separation and morphological method,then the inhibiting effect of three kinds of fungicides on the pathogen were studied by AGAR mycelia growth inhibition method.The results showed that pathogen isolated from Hengyang city was identified as Corynespora viticis,50% carbendazim and 75% chlorothalonil had better inhibition effects on it,EC50 were 1.782 and 2.080 g.L-1 respectively,which could be used.
参考文献/References:
[1]孙晓萍.金叶女贞的引种栽培和园林价值[J].浙江林学院学报,1995,12(2):156-160.
[2]林辰松,刘畅,王磊.金叶女贞在城市和园林绿化中的应用[J].山西建筑,2010,36(14):352.
[3]王国良.金叶女贞假尾孢叶斑病初步研究[J].浙江万里学院学报,2000(2):14-16+8.
[4]王国良.金叶女贞轮纹病的初步研究[J].浙江农业科学,2000(4):37-39.
[5]蔡平,毛建萍,陆小燕,等.金叶女贞假尾孢病害的生物学特性和杀菌剂筛选[J].江苏林业科技,2005(2):5-7.
[6]何苏琴,金秀琳,王卫成.金叶女贞褐斑病病原鉴定[J].植物保护,2006(2):70-72.
[7]张猛,武海燕,裴洲洋,等.河南省金叶女贞一新病害[J].河南农业大学学报,2008(2):220-222.
[8]龙柏云,谢宝多,马白菡.株洲市金叶女贞主要病虫害发生规律及防治对策[J].湖南农业大学学报:自然科学版,2010(S2):167-169.
[9]方中达.植病研究方法[M].3版.北京:中国农业出版社,1988:25-27.
[10]Marais I.Efficacy of fungicides against Colletotrichum coccodes on potato tubers[J].Potato Research,1990,33:275-281.
[11]李玉中,杨芳,刘春,等.衡阳地区莴笋叶斑病病原菌的鉴定及其室内药剂筛选[J].江苏农业科学,2014,42(2):102-104.
[12]杨成德,姜红霞,陈秀蓉,等.甘肃省马铃薯炭疽病的鉴定及室内药剂筛选[J].植物保护,2012,38(6):137-133.
[13]郭兰英.棒孢菌属4个新种[J].真菌学报,1984,3(3):161-169.
[14]徐东生.农药防治金叶女贞褐斑病的研究[J].北方园艺,2009(4):99-101.
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备注/Memo
收稿日期:2014-03-22
基金项目:衡阳市科技局科技发展计划资助项目(2011KN12);衡阳师范学院大学生研究性学习和创新性实验计划资助项目(CX1316)
第一作者简介:李玉中(1978-),男,山东省聊城市人,博士,副教授,从事微生物及植物病害防治等教学与科研工作。E-mail:yzl_2002@163.com。