[1]李巧丹,习海涛,张运海,等.干细胞因子对小鼠附植前胚胎体外发育的影响[J].黑龙江农业科学,2014,(08):65-68.
　LI Qiao-dan,XI Hai-tao,ZHANG Yun-hai,et al.Effect of Stem Cell Factor on Ectogenesis of Preimplantation Embryo of Mice[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(08):65-68.
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干细胞因子对小鼠附植前胚胎体外发育的影响

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2014年08 页码: 65-68 栏目: 出版日期: 2014-08-10
Title:
Effect of Stem Cell Factor on Ectogenesis of Preimplantation Embryo of Mice
文章编号:
1002-2767（2014）08-0065-04
作者:
李巧丹1; 习海涛2; 张运海1; 3; 4; 李运生1; 3; 4; 方富贵1; 3; 4; 章孝荣1; 3; 4; 刘亚=
1.安徽农业大学 动物科技学院，安徽 合肥 230036；
2.温州医学院附属第二医院，浙江 温州 325000；
3.安徽地方畜禽遗传资源保护与生物育种省级实验室，安徽 合肥 230036；
4.安徽省羊繁育工程技术研究中心，安徽 合肥 230026
Author(s):
LI Qiao-dan1; XI Hai-tao2; ZHANG Yun-hai 1; 3; 4; LI Yun-sheng 1; 3; 4; FANG Fu-gui 1; 3; 4; ZHANG Xiao-rong 1; 3; 4; LIU Ya 1; 3; 4
1.College of Animal Sciences and Technology,Anhui Agricultural University,Hefei,Anhui 230036;
2.The Second Affiliated Hospital of Wenzhou Medical University,Wenzhou,Zhejiang 325000;
3.Anhui Provincial Laboratory for Local Livestock of Poultry Genetic Resource Conservation and Bio-Breeding,Hefei,Anhui 230036;
4.Engineering Research Center of Reproduction and Sheep Breeding in Anhui Province,Hefei,Anhui 230036
关键词:
干细胞因子; 小鼠; 胚胎; 体外培养
Keywords:
stem cell factor; mice; embryo; in vitro culture
分类号:
Q813
文献标志码:
A
摘要:
为优化胚胎体外培养体系，提高胚胎体外培养发育率和发育质量，以昆明小鼠体内受精胚、体外受精胚和孤雌激活胚为研究对象，研究不同浓度干细胞因子对小鼠不同来源胚胎体外发育的影响。结果表明：在KSOM培养液中，小鼠自然受精胚、体外受精胚和孤雌激活胚的卵裂率和桑椹胚率均无显著差异（P>0.05），但IVN胚和IVF胚的囊胚发育率及囊胚细胞总数显著高于孤雌激活胚；培养液中添加不同浓度的干细胞因子对小鼠自然受精胚和孤雌激活胚的卵裂率、桑椹胚率、囊胚发育率和囊胚细胞总数都没有显著的影响（P>0.05)，但干细胞因子浓度为100 ng.mL-1时，小鼠体外受精胚的桑椹胚率和囊胚发育率显著高于对照（P<0.05）。
Abstract:
In order to optimize in vitro culture system of embryo to improve development rate and quality，taking in vivo fertilization embryo，in vitro fertilization embryo and parthenogenetic activation as research object，the effect of different concentrations of stem cell factor on embryo culture in vitro were studied.The results showed that the cleavage rate and morula rate had no significant difference in KSOM,but the blastocyst rate and total blastocyst number derived from PA was less than IVN and IVF.Different concentrations of stem cell factor had no significant effect on cleavage rate,morula rate,blastocyst rate and total blastocyst number.When the concentration of stem cell factor was 100 ng?mL-1,the morula rate and blastocyst rate were significantly higher than CK.

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[1]王春花,张云龙.锦灯笼多糖对CCl4诱导的急性肝损伤小鼠的保肝活性[J].黑龙江农业科学,2019,(09):111.[doi:10.11942/j.issn1002-2767.2019.09.0111]
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备注/Memo

收稿日期：2014-04-11
基金项目：安徽省自然科学基金资助项目（090413088）；安徽省创新试点省资助项目（12z010244）
第一作者简介：李巧丹（1988-），女，湖北省随州市人，硕士，研究实习员，从事动物生殖生物技术研究。E-mail:qiao9950@126.com。
通讯作者：刘亚（1972-），男，安徽省亳州人，博士，副教授，从事动物繁殖技术研究。E-mail:liuya@ahau.edu.cn。

更新日期/Last Update: 2014-12-03