KONG Wei-long,WANG Guang-quan,MENG Qing-jie,et al.Primary Exploration about Tissue Culture of Fresh Hawthorn Mengshanhong[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(08):28-31.
鲜食山楂新品种蒙山红组织培养初探
- Title:
- Primary Exploration about Tissue Culture of Fresh Hawthorn Mengshanhong
- 文章编号:
- 1002-2767(2014)08-0028-04
- Keywords:
- hawthorn; Mengshanhong; Dajinxing; tissue culture
- 分类号:
- S661.5
- 文献标志码:
- A
- 摘要:
- 为获得适宜山楂蒙山红快速繁殖的最佳培养基组合,以山楂新品种蒙山红和大金星的当年生带芽茎段为外植体,设置8种培养基组合进行愈伤组织诱导和芽增殖培养,分析两个山楂品种的组织培养的最适培养条件。结果表明:蒙山红诱导愈伤组织的最适培养基是MS+KT 1.0 mg?L-1+IBA 0.1 mg.L-1,最适宜的芽诱导培养基是MS+KT 1.0 mg.L-1+IBA 0.5 mg.L-1。大金星诱导愈伤组织的最适培养基是MS+KT 0.5 mg.L-1+IBA 0.5 mg.L-1,最适宜的芽诱导培养基是MS+KT 1.5 mg.L-1+IBA 0.5 mg.L-1。全部培养基均附加蔗糖30 g.L-1,琼脂7 g.L-1,pH5.8。
- Abstract:
- In order to obtain the most appropriate rapid propagation medium of hawthorn variety of Mengshanhong,taking stems section from Mengshanhong and Dajinxing as explants.Eight kinds of medium combination on callus induction and bud multiplication culture were set up to analyze the optimum culture conditions of two hawthorn varieties.The results showed that the best culture medium for callus induction was MS+KT 1.0 mg.L-1+IBA 0.1 mg.L-1 for Mengshanhong,and the best culture medium for bud proliferation was MS+KT 1.0 mg.L-1+IBA 0.5 mg.L-1.The best culture medium for callus induction was MS+KT 0.5 mg.L-1+IBA 0.5 mg.L-1 for Dajinxing,and the best culture medium for bud proliferation was MS+KT 1.5 mg.L-1+IBA 0.5 mg.L-1.30 g.L-1 sugar and 7 g.L-1 agar were added to all culture medium,and pH was 5.8.
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相似文献/References:
[1]刘晓莉,姜少娟.山楂果胶提取工艺的研究[J].黑龙江农业科学,2014,(10):107.
LIU Xiao-li,JIANG Shao-juan.Optimum Extraction Process of Pectin from Hawthorn[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(08):107.
备注/Memo
收稿日期:2014-03-07
基金项目:大学生科技文化创新基金资助项目(理工类SF2012155);农业科技成果基金资助项目[鲁科农(2012)59];山东省科技攻关资助项目(2009GG10009031)
第一作者简介:孔维龙(1991-),男,山东省济宁市人,学士,在读硕士,从事园艺植物资源开发和利用研究。E-mail:kongweilong23@gmail.com。
通讯作者:王光全(1957-),男,学士,教授,从事园艺植物种质资源研究。E-mail:wgq@lcu.edu.cn。