[1]董佩佩,康涛,孙漫红,等.粉红粘帚霉67-1厚垣孢子形成与贮存研究[J].黑龙江农业科学,2013,(10):48-50.
　DONG Pei-pei,KANG Tao,SUN Man-hong,et al.Formation and Storage of Clonostachys rosea 67-1 Chlamydospores[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(10):48-50.
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粉红粘帚霉67-1厚垣孢子形成与贮存研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2013年10 页码: 48-50 栏目: 出版日期: 2013-10-31
Title:
Formation and Storage of Clonostachys rosea 67-1 Chlamydospores
文章编号:
1002-2767（2013）10-0048-03
作者:
董佩佩1; 2; 康涛1; 孙漫红2; 李世东2; 罗明1
1.新疆农业大学 农学院，新疆 乌鲁木齐 830052;
2.中国农业科学院 植物保护研究所/农业部作物有害生物综合治理综合性重点实验室,北京 100081
Author(s):
DONG Pei-pei 1; 2; KANG Tao1; SUN Man-hong2; LI Shi-dong2; LUO Ming1
1.College of Agronomy,Xinjiang Agricultural University,Urumqi,Xinjiang 830052;
2.Key Laboratory of Integrated Pest Management in Crops,Ministry of Agriculture,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100081
关键词:
粉红粘帚霉; 厚垣孢子; 货架期; 生物防治
Keywords:
Clonostachys rosea; chlamydospores; shelf life; biological control
分类号:
S476+.1
文献标志码:
A
摘要:
为研制粘帚霉生防制剂，以粉红粘帚霉67-1为材料，对粉红粘帚霉67-1厚垣孢子的生长过程、孢子结构、贮存介质和制剂含水量以及厚垣孢子和分生孢子的货架期比较进行了研究。结果表明:在特定的培养基中，粉红粘帚霉67-1在培养24 h后产生厚垣孢子，厚垣孢子形成于菌丝顶端或中部，并可以脱落游离到培养基中。电镜下可以看到圆形或椭圆形厚垣孢子以及加厚的细胞壁。相同贮存条件下，厚垣孢子的货架期比分生孢子长。低温条件下，10%含水量的贮存介质更利于厚垣孢子的贮存。
Abstract:
In order to develop Gliocladium biocontrol preparation,taking Clonostachys rosea 67-1 chlamydospores as test materials,the growth process,morphology,shelf life,storage media,water content and comparation between chlamydospores and conidia were studied.The results showed that chlamydospores produced in specific medium after culturing 24 hours，chlamydospores formed in the centre of hyphae or at the top,and it could fall off into the medium.Circular or ellipse thick wall structure and incrassate cell wall could be seen under the electron microscope.In the same storage conditions,chlamydospores had longer shelf life than condia.Under low temperature,the storage medium of 10% water content could extend shelf life of chlamydospores of Clonostachys rosea 67-1.

参考文献/References:

[1] Kubicek C P,Harmn G E.Trichoderma and Gliocladium[M].Padstow Taylor and Francis Ltd.Press,UK:London,1998:193-200.
[2]Sharma B K,Singh B M.Biological control of white rot of pea caused by Sclerotinia sclerotiorum(Lib1)de Bary[J].Journal of Biological Control,1990,4(2):132-134.
[3]张拥华,高会兰,马桂珍,等.粉红粘帚霉67-1菌株寄生核盘菌研究[J].植物病理学报,2004,34(3):211-214.
[4]暴增海,吴雪仁,杨文兰,等.粘帚霉(Gliocladium spp.)不同菌株对几种病原菌的抑菌作用测定[J].吉林农业大学学报,2004,26(4):394-398.
[5]马桂珍,高会兰,张拥华.链孢粘帚霉几丁质酶的诱导及其抗真菌活性研究[J].微生物学通报,2007,34(5):905-908.
[6]Lewis J A,Papavizas G C.Production of chlamydospores and conidia by Trichoderma spp.in liquid and solid growth media[J].Soil Biology and Biochemistry,1983,15(3):351-357.
[7]李卫平,王洪凯,林福呈.稻曲病菌厚垣孢子萌发特性研究[J].浙江农业学报,2008,20(4):278-281.
[8]杨秀娟,王舒婷,甘林,等.稻曲病菌生长及其分生孢子萌发特性[J].福建农林大学学报,2011,40(6):576-581.
[9]Beagle-Ristaino J E,Papavizas G C.Survival and proliferation of Trichoderma spp.and Gliocladium virens in soil and in plant rhizospheres[J].Phytopathology,1985,75(6):729-732.
[10]潘玮.绿色木霉厚垣孢子与分生孢子生物学特性及生防效果的比较研究[D].北京:中国农业科学院,2006:8-9. [11]张拥华,李世东,王桂琴,等.粘帚霉可湿性粉剂防治大豆菌核病试验[J].植物保护,2007,33(5):141-142.
[12]Hughes S J.The term chlamydospore.In:Tadashi A,Tetsurō K(eds.)Filamentous microorganisms[M].Japan Scientific Societies Press,Japan:Tokyo,1985：1-20.
[13]Cliquet S,Ash G,Cother E.Production of chlamydospore and conidia in submerged culture by Rhynchosporiumalismatis,a mycoherbicide of Alismataceae in rice cope[J].Biocontrol Science and Technology,2004,14(8):801-810.
[14]张丽靖,冯明光.基于球孢白僵菌的真菌杀虫剂生产工艺与剂型述评[J].浙江农业学报，2004,16(6):395-399. [15]冯明光,应盛华.不同含水量和温度下贮存中球孢白僵菌分生孢子活力与内贮营养的衰变[J].应用生态学报,2002,13(4):439-443.

备注/Memo

收稿日期：2013-06-27
基金项目：国家“863”计划资助项目（2011AA10A205）；国家“十二五”科技支撑计划资助项目（2012BAD19B01）；行业技术体系资助项目（Nycytx-35-gw29）
第一作者简介：董佩佩（1986-），女，河北省保定市人，在读硕士，从事植物病害生物防治研究。Email：peipeidong2006@163.com。

更新日期/Last Update: 2014-11-13