HU Chun-hui,LU Wan-pei,LIU Peng-qi,et al.Study on Sampling Methods of Scanning Electron Microscope on Pollen and Bud of Geranium tsingtauense Yabe.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(12):82-84.
青岛老鹳草花粉和花芽扫描电镜制样条件探讨
- Title:
- Study on Sampling Methods of Scanning Electron Microscope on Pollen and Bud of Geranium tsingtauense Yabe.
- 文章编号:
- 1002-2767（2013）12-0082-03
- Keywords:
- scanning electron microscope(SEM); Geranium tsingtauense Yabe.; pollen; flower bud; specimen preparation
- 分类号:
- Q-336
- 文献标志码:
- A
- 摘要:
- 为了探究与老鹳草相关类型花粉花芽的样品电镜制备，采用直接观察法和戊二醛固定冷冻干燥法对青岛老鹳草花粉扫描电镜制样条件进行了研究。结果表明：直接观察法制备的青岛老鹳草花粉颗粒在扫描电镜下形态饱满，结构清晰，花粉表面的纹饰和突起微观结构清晰完整；改进的FAA单固定冷冻干燥法处理的青岛老鹳草花芽形态学结构完整，层次丰富，立体感强，细胞失水皱缩得到明显改善。通过对不同制样方法进行比较，最终得到可靠简单易行的样品制备方法，对牻牛儿苗科植物的研究及相关类型花粉花芽的样品制备有重要的指导意义。
- Abstract:
- In order to explore the revelant types of Geranium pollen and flower buds preparation methods of scanning electron microscope,air-dried and glutaric aldehyde fixed methods were compared to study preparation condition of scanning electron microscopy.The results showed that Geranium pollen grains prepared through direct observation were full and clear in the scanning electron microscopy,microscopic structure were clear and morphological integrity;micro-structure of flower bud treated with FAA single fixation were morphological integrity,layers rich,stereo sense strong,cell dehydration shrinkage was improved significantly.Through the comparison of different specimen preparation methods,reliable technique which was easier in operation and low cost was achieved,it had important guiding significance for the preparation of Geranium tsingtauense.pollen and bud.
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备注/Memo
收稿日期：2013-08-23
基金项目：青岛农业大学实验技术研究资助项目（SYJK12-03）
第一作者简介：胡春辉（1987-），女，山东省潍坊市人，硕士，助理实验师，从事扫描电镜的使用和维护方面的研究。E-mail：hollyhuchunhui@gmail.com。
通讯作者：袁玉清（1965-），男，山东省荣成市人，学士，高级实验师，从事仪器分析方面的研究。E-mail：sbk@qau.edu.cn。