LIU Chun-ying,FENG Ying,SHI Yu-ping,et al.The Determination of Free Amino Acid Content in Rape Bee Pollen[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(10):122-124.
油菜蜂花粉中游离氨基酸含量的测定
- Title:
- The Determination of Free Amino Acid Content in Rape Bee Pollen
- 文章编号:
- 1002-2767（2012）10-0122-03
- 关键词:
- 蜂花粉; 氨基酸; 双光束紫外分光光度计
- Keywords:
- bee pollen; amino acids; double beam UV spectrophotometer
- 分类号:
- TS201
- 文献标志码:
- A
- 摘要:
- 用双光束紫外分光光度计测定油菜蜂花粉中游离氨基酸的含量。在该过程中，花粉用43°青稞白酒提取，提取液先用氢氧化钡沉淀除去色素和蛋白质，再用水合茚三酮显色，通过双光束紫外分光光度法测氨基酸含量。结果表明：在相同质量的花粉中，未破壁油菜花粉中游离氨基酸的含量为1.87％～4.91％，破壁花粉中游离氨基酸的含量为2.46％～8.26％。该方法中的仪器易于操作，测定结果准确可靠。
- Abstract:
- The content of free amino acids in the rape bee pollen was determined by the double beam ultraviolet spectrophotometry.In the process,samples were extracted with 43 degrees of the barley wine.Pigment and protein were precipitated out by Ba(OH)2,Ferrocenylmethyl-substituted indenes three ketone was put in the filtrate and shown the color.The results showed that the content of free amino acid was in the 1.87% to 4.91% range in bee pollen failed to break the wall,and the content of free amino acid was in the 2.46% to 8.26% range in bee pollen broken the wall.The method of the instrument was easy to operate and the result accurate and reliable.
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备注/Memo
基金项目： 青海省科技厅资助项目（2011-G-401）