SUN Ying,HOU Zhi-xia.Comparison of Methods for Total RNA Extraction from Blueberry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(10):114-117.
蓝莓果实总RNA提取方法比较
- Title:
- Comparison of Methods for Total RNA Extraction from Blueberry
- 文章编号:
- 1002-2767（2012）10-0114-04
- Keywords:
- blueberry; total RNA; extraction methods
- 分类号:
- S663.9
- 文献标志码:
- A
- 摘要:
- 为了在蓝莓果实中提取出高质量RNA，比较了改良CTAB法、TRIzol法、试剂盒法和SDS法4种方法。结果表明：改良CTAB大量法能有效地去除果实中的多糖和酚类物质，获得的总RNA质量较好，纯度较高。28s∶18s的亮度约为2∶1，OD260/OD280在1.8～2.0，OD260/OD230的比值都大于2.0，且得率也较高，幼果期为23.978 μg·g-1，完熟期为30.817 μg·g-1。完全能适用于RT-PCR、转录组测序等分子生物学实验。
- Abstract:
- In order to extract high quality RNA from blueberry,modified CTAB,modified SDS,Trizol Kit and total RNA extraction kit were used to extract total RNA from blueberry fruits of different development stage.The results showed that modified CTAB was considered to be the most favorable methods for RNA isolation from blueberry,RNA with high yield and quality.Following this efficient procedure,we routinely obtained 23.978 μg·g-1 total RNA from young fruit stage and 30.817 μg·g-1total RNA from full maturity stage,the brightness of 28s∶18s was about 2∶1,the ratios of OD260/OD280=1.8～2.0 and OD260/OD230>2.0.The RNA was suitable for some biotechnological researchers,such as RT-PCR and transcription sequencing.
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备注/Memo
基金项目： 林业公益性行业科研专项资助项目（200904014）；北京林业大学森林培育科技创新平台开放基金资助项目（000-1108008）