WANG Ming-quan.The Rapid Detection of Maize Germplasm ContainingTransgenic ?Bt Gene Using Bt Test Paper[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(10):21-24.
利用Bt试纸对转Bt基因玉米种质的快速检测
- Title:
- The Rapid Detection of Maize Germplasm ContainingTransgenic ?Bt Gene Using Bt Test Paper
- 文章编号:
- 1002-2767（2012）10-0021-04
- Keywords:
- maize; Bt test paper; transgenic Bt gene; maize germplasm
- 分类号:
- S513
- 文献标志码:
- A
- 摘要:
- 玉米是世界三大粮食作物之一，更是重要的饲料作物，并且在世界粮食生产中处于重要的位置，因此转基因玉米的研究和发展受到广泛关注。如何更加安全准确地检测玉米中是否含有转基因，是许多学者一直研究的内容。利用Bt-Cry1Ac/Ab试纸对转Bt基因玉米种质进行检测，初步筛选出含有转Bt基因的玉米种质，同时分析了影响Bt-Cry1Ac/Ab试纸检测的因素。
- Abstract:
- The study and development of the transgenic maize has been received the important attention because maize is one of the three major foods,important forage crop and occupies an important position for the food production in the world.How to safe and accurate detection of maize with or without the transgene attracts the attention of many researchers.In the work,maize germplasm containing transgenic Bt gene were initially screened out by means of Bt-Cry1Ac/Ab test paper，meanwhile,the factors affcting Bt-Cry1Ac/Ab test paper were annlyzed.
参考文献/References:
[1]闫新甫，转基因植物[M].北京:科学出版社,2003：257-277. [2]蔡泉，曹靖生，史桂荣，等.利用Bt试纸快速检测玉米中的Bt蛋白[J].黑龙江农业科学,2010（10）：133-134. [3]黄敏，杜何为，张祖新，等.转Bt基因玉米杂交种的快速检测技术[J].湖北农业科学,2004(5):39. [4]李桂玲，李明泽，刘宗华，等.转Bt基因抗虫玉米的研究[J].安徽农业科学,2007,35(13):3376-3801.
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备注/Memo
作者简介： 王明泉（1978-），男，黑龙江省通河县人，硕士，副研究员，从事玉米遗传育种研究。E-mail：wangmingquan 8888@yahoo.com.cn。