ZHANG Dao-wei,CHEN Jing,GUO Yu-shuang.Cloning and Characterization Analysis of Heat ShockProtein 90 Gene from Sogatella furcifera[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(06):13-18.
白背飞虱HSP90全长cDNA的克隆与特性分析
- Title:
- Cloning and Characterization Analysis of Heat ShockProtein 90 Gene from Sogatella furcifera
- 文章编号:
- 1002-2767（2012）06-0013-06
- 分类号:
- S435.112.3
- 文献标志码:
- A
- 摘要:
- 利用同源克隆及RACE的方法，从白背飞虱Sogatella furcifera中克隆获得热激蛋白HSP90基因的全长cDNA序列(Genbank登录号：JQ743628)，该基因全长为2 707 bp，包含421 bp的3′非编码区域(UTR)和93 bp的5′UTR，开放阅读框(ORF)为2 193 bp，编码730个氨基酸。该基因预测的蛋白分子量为83.85 kD，等电点为4.94，无信号肽、糖基化位点及跨膜结构。在N端具有HSP90基因保守的ATPase结构，含有HSP90家族的C末端的保守序列MEEVD。从进化分析结果看出，白背飞虱的HSP90与其它昆虫的HSP90蛋白的同源性较高，与褐飞虱相比较，同源性高达98%。HSP90基因的克隆和比较分析为进一步深入研究白背飞虱的抗逆机理、耐受性及进化具有重要意义。
- Abstract:
- The heat shock protein 90(HSP90)gene from Sogatella furcifera was cloned by homology cloning strategy and technique of Rapid Amplification of cDNA Ends(RACE).The full-length cDNA of S.furcifera HSP90(Sf HSP90) is 2 707 bp(Genbank number：JQ743628)and has an open reading frame of 2 193 bp,a 3 UTR(untranslated region)of 421 bp and a 5UTR of 93 bp.The estimated molecular weight of HSP90 is 83.85 kD and pI is 4.94.There are no glycosylation sites,putative transmembrane domain and signature peptide.It contains ATPase conservative domain in N-site and MEEVD signature sequence on C-site.Compared with Nilaparvata lugens,they are 98％ identities and Phylogenetic analysis shows that these two species are most closely related.Cloning and comparison analysis of HSP90 genes from S.furcifera could be useful in the studies of anti-stress mechanism,tolerance improvement breeding and evolution for these animals.
参考文献/References:
[1]Rutherford S L,Zuker C S.Protein folding and the regulation of signaling pathways[J].Cell,1994,79(7):1129 - 1132. [2]Borkovich K A,Farrelly F W,Finkelstein D B,et al.Hsp82 is an essential protein that is required in higher concentrations for growth of cells at higher temperatures[J].Molecular and Cellular Biology,1989,9(9):3919-3930. [3]Ritassa F M.Experimental activation of specific puffloci in polytene chromosomes of Drosophila[J].Experimental Cell Research,1964,35:601-607. [4]Ritossa F M.A new puffing pattern induced by heat shock and DNP in Drosophilia[J].Experientia,1962,18:571-573. [5]Dean R L,Atkinson B G.The acquisition of thermaltoler ancein larvae of Calpodes ethius (Lepidoptera)and the situandin vitro synthesis of heat-shock protein[J].Can J Biochem Cell Biol,1982,61:472-479. [6]Whyard S,Wyatt G R,Walker V K.The heat shock response in Locustami gratoria[J].J Comp Physiol,1986,156:813-817. [7]Baldaia L,Maisonhaute C,Porcheron P,et al.Effect of heat shock on protein synthesis in Locustami gratoria epidermis[J].Arch Insect Biochem Physiol,1987(4):225-231. [8]Ruder G K,Ovsenek N,Heikkila J J,et al.Examination of heat shock gene expression in nerve cord isolated from heat-stressed American cockroach,Periplaneta americana,Biochem[J].Cell Biol,1989,67:168-178. [9]王荫长.昆虫生物化学[M].北京:中国农业出版社,2001. [10]李斌，夏庆友，孙卫忠，等.昆虫HSP基因的系统研究[J].蚕业科学,2003,29(4):340-343. [11]Huang L H,Wang C Z,Kang L.Cloning and expression of five heat shock protein genes in relation to cold hardening and development in the leafminer,Liriomyza sativa[J].J Insect Physiol.2009,55(3):279-285. [12]Prodromou C,Roe S M,O’Brien R,et al.Identification and structural characterization of the ATP/ADP-binding site in the Hsp90?molecular chaperone[J].?Cell,1997(a),90(1):65-75. [13]Prodromou C,Roe S M,Piper P W,et al.A molecular clamp in the crystal structure of the N-terminal domain of the yeast Hsp90 chaperone[J].Nature Structural Bio1ogy,1997(b),4(6):477-482. [14]Stebbins C E,Russo dA A,Schneider C,et al.Crystal structure of an Hsp90-geldanamycin complex:Targeting of a protein chaperone by an antitumor agent[J].Cell,1997,89(2):239-250. [15]Peter C,Tamas S,Csaba S.The 90-kDa molecular chaperone family:Structures function and clinical applications.A comprehensive review[J].Pharmacology Therapeutics,1998,79(2):129-168.
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备注/Memo
第一作者简介： 张道伟（1981-），男，河南省内乡县人，博士，讲师，从事昆虫及农业螨类分子生物学研究。E-mail：zhangdw1000@163.com。