WANG Guan,WANG Ming-yu,MU Zi-ming,et al.Optimization of Cellulose Production by Trichoderma reesei[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(05):108-111.
里氏木霉纤维素酶生产工艺的优化
- Title:
- Optimization of Cellulose Production by Trichoderma reesei
- 文章编号:
- 1002-2767（2012）05-0108-04
- Keywords:
- Trichoderma reesei; cellulose; pH; temperature; optimization
- 分类号:
- Q815
- 文献标志码:
- A
- 摘要:
- 提高纤维素酶生产效率，降低纤维素酶生产成本是纤维素乙醇生产技术的关键之一。而碳源、氮源和无机盐等产酶培养基成分以及接种时间、产酶温度、培养初始pH等产酶条件是纤维素酶生产过程中的关键因素。为充分利用里氏木霉生产纤维素酶，研究了纤维素酶高产菌株里氏木酶FST-1产酶培养基和产酶条件对纤维素酶产酶的影响。结果表明：麸皮、蛋白胨和磷酸二氢钾的含量对于纤维素酶的生产影响较大，并且确定了最优产酶培养基为4号培养基。通过对不同产酶条件的研究，确定最佳接种时间为24 h、最佳产酶温度为32℃、最佳初始pH为5.5，优化后的生产工艺可以将滤纸酶活力和蛋白含量提高3倍。
- Abstract:
- Reducing cellulose production cost through improving cellulose titers during the fermentation is a key strategy for developing economically competitive bioethanol production from lignocellulosic biomass.Medium composition and inoculate time,temperature,initial pH and other factors were key factors to cellulose production.For the purpose to fully used Trichoderma reesei to produce cellulose,the influence of medium composition and growth condition of the cellulose-producing strain Trichoderma reesei FST-1 on its enzyme production was investigated.The result showed that the contents of wheat bran,peptone and KH2PO4 had the significant effect on cellulose titer,the No.4 medium was the best medium.Through the study on different enzyme production condition,it found that the optimum inoculation time was 24 hours,the optimum temperature was 32℃ and the optimum initial pH was 5.5.Cellulose titer and soluble protein content were increased 3 times with optimized culture condition.
参考文献/References:
[1] 张艾莉，王成军 . 燃料乙醇工业的发展对我国环境保护的贡献 [J]. 全球科技经济瞭望 ,2007(2):54 - 55. [2]郭平.多因素影响纤维沥青胶浆流变性能研究[J].广西大学学报：自然科学版，2010,35(1):105-109. [3]邓勇，房俊民，陈方，等.生物燃料最新发展态势分析[J].中国生物工程,2008,28(8):142-147. [4]冯健玲，姚晓华，韦秉兴，等.稻草秸秆纤维素分解菌的分离筛选[J].基因组学与应用生物学，2009，28（3）：477-480. [5]Qu Yinbo，Zhu Mingtian，Liu Kai，et al.Studies on cellulosic ethanol production for sustainable supply of liquid fuel in China[J].Biotechnol Journal，2006,1(1):1235-1240. [6]Fang Xu，Shen Yu，Zhao Jian，et al.Status and prospect of lignocellulosic bioethanol production in China[J].Bioresoure Technology，2010,101(13):4814-4819. [7]匡廷云，白克智，杨秀山.我国生物质能发展战略的几点意见[J].化学进展，2007,19(7):1060-1063. [8]Palmqvist E，Hahn-Hagerdal B.Fermentation of lignocellulosic hydrolysatesⅡ：inhibitors and mechanisms of inhibition[J].Bioresoure Technology，2000,74(1)：25-33. [9]Liu Kai，Lin Xiaohui，Yue Jun,et al .High concentration ethanol production from corncob residues by fed-batch strategy[J].Bioresoure Technology，2010,101(13)：4952-4958. [10]Ouyang Jia，Dong Zhenwei，Song Xiangyang,et al.Improved enzymatic hydrolysis of microcrystalline cellulose(Avicel PH101)by polyethylene glycol addition[J].Bioresoure Technology，2010,101(17)：6685-6691. [11]Zhang Mingjia，Su Rongxin，Qi Wei,et al .Enhanced enzymatic hydrolysis of lignocellulose by optimizing enzyme complexes[J].Applied Biochemistry and Biotechnology，2010,160(5)：1407-1414. [12]Wang Mingyu，Li Zhonghai，Fang Xu，et al.Cellulolytic enzyme production and enzymatic hydrolysis for second-generation bioethanol production[J].Advances in Biochemical Engineering/Biotechnology,2012,DOI:10.1007/10_2011_131. [13]Himmel M E，Ding S Y，Johnson D K，et al.Biomass recalcitrance engineering plants and enzymes for biofuels production[J].Science，2007,315(5813):804-807. [14]Sanchez C.Lignocellulosic residues：biodegradation and bioconversion by fungi[J].Biotechnology Advance，2009,27(2)：185-194. [15]张辉，桑青.响应面法优化黑曲霉HQ-1产纤维素酶固体发酵条件[J].中国酿造，2011（7）：17-22. [16]Ikeda Y,Hayashi H,Okuda N,et al .Efficient cellulase production by the filamentous fungus Acremonium cellulolyticus[J].Biotechnol Prog，2007，23（2）：333-338. [17]Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Analytical biochemistry,1976,72(1-2):248-254. [18]Yamanobe T，Mitsuishi Y，Takasaki Y.Isolation of a cellulolytic enzyme producing microorganism,culture conditions and some properties of the enzyme[J].Agricultural Biology and Chemistry，1987,51 (1)：65-74. [19]刘凯，林建强，曲音波.纤维素酶生产技术[J].生物产业技术,2008,1(4):54-60.
相似文献/References:
[1]陈雪丽,迟凤琴,易晓东,等.纤维素降解菌群TF18的作用机制[J].黑龙江农业科学,2021,(02):41.[doi:10.11942/j.issn1002-2767.2021.02.0041]
[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(05):41.[doi:10.11942/j.issn1002-2767.2021.02.0041]
备注/Memo
基金项目：国家重点基础研究发展计划“973”资助项目（2011CB707403）；科技部国际科技合作计划资助项目（2010DFA32560）；新世纪优秀人才支持计划（NECT）资助项目