QU Zhen,CHANG Li-ming.Primary Study on Tissure Culture of NorthAmerican Begonia in Xining Area[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(09):11-12,29.
西宁地区北美海棠初代组织培养技术研究
- Title:
- Primary Study on Tissure Culture of NorthAmerican Begonia in Xining Area
- 文章编号:
- 1002-2767（2011）09-0011-02
- 分类号:
- S682.1+9
- 文献标志码:
- A
- 摘要:
- 选取北美海棠王族和绚丽两个品种为试材，以无性繁殖的方式选择外植体，接种于不同激素配比的MS培养基上，探究其初代组织培养技术。结果表明：无性繁殖选择以春季萌发的新梢或根部萌发的新枝条作为外植体适合不定芽诱导，其中诱导的最佳培养基为MS+6-BA1.0 mg·L-1+IBA0.3 mg·L-1；不定芽增殖最佳培养基为MS+6-BA1.6 mg·L-1+NAA0.2 mg·L-1。
- Abstract:
- Two species(Malus’Royally’,Malus’Radiant’）of North American Begonia were selected as materials,explants were selected by asexual reproduction way,and different hormones were inoculated on MS medium.The results showed that: clonal selection in the spring shoot or root germination of new shoots were suitable for using as explants for adventitious bud induction,the optimal medium was MS+6-BA1.0 mg·L-1+IBA0.3 mg·L-1;the optimal medium for adventitious bud proliferation was MS+6-BA1.6 mg·L-1+NAA0.2 mg·L-1.
参考文献/References:
[1]吴殿呈，胡繁荣．植物组织培养 [M]. 兰州：上海交通大学出版社， 1996. [2]曹春英．植物组织培养[M].北京：中国农业出版社，2005. [3]张天麟．园林树木1200种[M].北京：中国建筑工业出版社，2004. [4]陶绿.林业实用技术[M].北京：《林业实用技术》编辑部,2011.
相似文献/References:
[1]兰 伟,万凌云,马宗新,等.三个草莓品种的组织培养研究[J].黑龙江农业科学,2012,(08):21.
LAN Wei,WAN Ling-yun,MA Zong-xin,et al.Study on Tissue Culture of Three Varieties of Strawberry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(09):21.
[2]程密密,杨霞,杨玉灿,等.翠菊品种库蕾娜叶片和叶柄及茎段的再生研究[J].黑龙江农业科学,2014,(10):20.
CHENG Mi-mi,YANG Xia,YANG Yu-can,et al.Regeneration Research of Leaves,Petioles and Stem Segments of Callistephus chinensis Variety Kurenai[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(09):20.
[3]张天静,雷家军,孙文松.菊花品种神马的茎段离体快繁研究[J].黑龙江农业科学,2014,(12):19.
ZHANG Tian-jing,LEI Jia-jun,SUN Wen-song.Study on Stem Micropropogation of Chrysanthemum cv.Jinba[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(09):19.
[4]王小国.灵宝大枣无菌外植体的建立及茎段不定芽的诱导[J].黑龙江农业科学,2015,(05):13.[doi:10.11942/j.issn1002-2767.2015.05.0013]
WANG Xiao-guo.Establishment of Sterile Explant and Induction of Adventitious Bud from Stem of Lingbao Jujube[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(09):13.[doi:10.11942/j.issn1002-2767.2015.05.0013]
[5]张 波,石建宁,孙 东,等.四种北美海棠在银川市的引种驯化研究[J].黑龙江农业科学,2015,(06):91.[doi:10.11942/j.issn1002-2767.2015.06.0091]
ZHANG Bo,SHI Jian-ning,SUN Dong,et al.Introduction and Domestication of Four Species of Malus sectabilis Ait Borkh in Yinchuan[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(09):91.[doi:10.11942/j.issn1002-2767.2015.06.0091]
[6]何易航,黄三印,邓凯元,等.不同浓度三种激素对小蓟茎段愈伤组织诱导影响[J].黑龙江农业科学,2015,(09):26.[doi:10.11942/j.issn1002-2767.2015.09.0026]
HE Yi hang,HUANG San-yin,DENG Kai-yuan,et al.Effect of Different Concentrations of Three Kinds of Hormone on ?Callus Induction of ?Cirsium setosum Stem[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(09):26.[doi:10.11942/j.issn1002-2767.2015.09.0026]
[7]蒋瑶,陈文波,周朝伟,等.野生湖北百合不定芽诱导及再生植株的建立[J].黑龙江农业科学,2016,(09):11.[doi:10.11942/j.issn1002-2767.2016.09.0011]
JIANG Yao,CHEN Wen-bo,ZHOU Chao-wei,et al.Study on Adventitious Buds Induction and Plant Regeneration of Wild Liliumhenryi[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(09):11.[doi:10.11942/j.issn1002-2767.2016.09.0011]
[8]蒋 瑶,陈文波,周朝伟,等.野生湖北百合不定芽诱导及再生植株的建立[J].黑龙江农业科学,2016,(10):13.[doi:10.11942/j.issn1002-2767.2016.10.0013]
JIANG Yao,CHEN Wen-bo,ZHOU Chao-wei,et al.Adventitious Buds Induction and Plant Regeneration of Wild Lilium henryi[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(09):13.[doi:10.11942/j.issn1002-2767.2016.10.0013]
[9]康哲秀,吴京姬,郎贤波,等.马铃薯延薯4号再生体系建立[J].黑龙江农业科学,2016,(12):11.[doi:10.11942/j.issn10022767.2016.12.0011]
KANG Zhexiu,WU Jingji,LANG Xianbo,et al.Establishment of the Regeneration System of Potato Variety Yanshu 4[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(09):11.[doi:10.11942/j.issn10022767.2016.12.0011]
[10]白禹萱,蒲东祥,李阳,等.大樱桃Gisela矮化砧组织培养技术研究[J].黑龙江农业科学,2020,(07):87.[doi:10.11942/j.issn1002-2767.2020.07.0087]
[J].HEILONGJIANG AGRICULTURAL SCIENCES,2020,(09):87.[doi:10.11942/j.issn1002-2767.2020.07.0087]
备注/Memo
第一作者简介：瞿珍（1986-），女，甘肃省兰州市人，助理工程师，从事培养基制作及组培繁育研究。E-mail：443027475@qq.com。