[1]赵占鳌.蜜蜂幼虫冷冻技术研究[J].黑龙江农业科学,2010,(08):106-107.
　ZHAO Zhan-ao.Research on Freezing Technology of Bee Larva[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(08):106-107.
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蜜蜂幼虫冷冻技术研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2010年08 页码: 106-107 栏目: 出版日期: 2010-08-10
Title:
Research on Freezing Technology of Bee Larva
文章编号:
1002-2767(2010)08-0106-02
作者:
赵占鳌
黑龙江省农业科学院 牡丹江分院，黑龙江 牡丹江157041
Author(s):
ZHAO Zhan-ao
Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences，Mudanjiang，Heilongjiang 157041
关键词:
西方蜜蜂; 幼虫; 冷冻; 玻璃化
Keywords:
Apis mellifera; larva; freezing; vitrification
分类号:
S89
文献标志码:
A
摘要:
以西方蜜蜂幼虫为材料，采用玻璃化冷冻方法，研究了蜜蜂幼虫冷冻技术。结果表明：从多种抗冷冻保护液中筛选出的适宜蜜蜂幼虫的冷冻保护液为15%甘油+0.2 mol·L-1蔗糖+0.2 mol·L-1木糖+3%聚乙二醇+15%乙二醇。冷冻剂类为纱布包裹型。冷冻程序：预冷时间为5 min，距液氮高度2~7 cm，降速率为1 cm·min-1。解冻液为生理盐水+葡萄糖。此方法冷冻幼虫解冻后成活率为10%左右。
Abstract:
Bee larva freezing technology is based on Apis mellifera which makes reference to the embryo freezing technology of large livestock，using vitrification freezing technology.After repeated experiments，the suitable bee larva freezing protective liquid which was selected from a lot of anti-freeze protective liquid was:15% glycerol+0.2 mol·L-1sucrose+0.2 mol·L-1 xylose+3%polyethyleneglycol+15%glycol.The type of cryogen was gauze wrapping.Frozen procedure:pre-cooling time was 5 min，the height above the liquid nitrogen surface was 2~7 cm，the decreasing rate was 1cm·min-1.The thawing solution was physiological saline+ glucose.The survival rate of the freezing larva was about 10% by using this method.

参考文献/References:

[1]周冰峰，林世煌，苏静，等 . 温度对蜜蜂受精卵和封盖王蛹发育的影响[J].福建农林大学学报 ( 自然科学版 )，2002(4):511 - 513.

[2] Yokota Y，Sato S，Yokoto M，et al.Birth of a healthy baby following vitrification of human blastocysts[J].Fertil Steril,2001,75(5):1027-1029.

[3] 罗晓中，杨志明.组织工程化组织的低温冷冻保存研究[J].中国临床康复，2004，17(8):3327-3329.


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备注/Memo

作者简介：赵占鳌(1960-)，男，黑龙江省牡丹江市人，农艺师，从事蜜蜂饲养管理与育种工作。E-mail:lvyunling1963@126.com。

更新日期/Last Update: 2014-11-06