[1]宋 洋,李柱刚.DGGE技术在土壤微生物群落研究中的应用[J].黑龙江农业科学,2010,(07):5-8.
 SONG Yang,LI Zhu-gang.Application of DGGE in Soil Microorganism[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(07):5-8.
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DGGE技术在土壤微生物群落研究中的应用

参考文献/References:

[1]郝文英 . 中国农业百科全书:土壤卷[M] . 北京:农业出版社, 1996 385 - 400 .

[2]焦晓丹,吴凤芝.土壤微生物多样性研究方法的进展[J].土壤通报,200435(6)789-792.

[3]Brock T D.The study of microorganisms in situprogress and problems[J].Symp Soc Gene Microbial1987411-17.

[4]Muyzer GSmalla K.Application of denaturing gradient gel electrophoresis(DGGE)and temperature gradient gel electrophoresis(TGGE)in microbial ecology[J].Antonie van Leeuwenhoek199873127-141.

[5]Muyzer Gde Waal E CUitterlinden A G.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA[J].Appl Environ Microbiol199359:695-700.

[6]Muyzer G.DGGE/TGGE a method for identifying genes from natural ecosystems [J].CurrOpin Microbiol19992(3)317-322.

[7]Fischer S GLerman L S.DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gelscorrespondence with melting theory[J].Proc National Acad Sciences USA198380(6):1579-1583.

[8]Lerman L SFischer S GHurley Iet al.Sequence-determined DNA separations[J].Annu Rev Biophys Bioeng198413399-423.

[9]Ma W KSiciliano S DGermida J J.PCR - DGGE method for detecting arbuscular mycorrhizal fungi in cultivated soils[J].Soil Biology and Biochemistry200537:1589-1597.

[10]Myers R MFischer S GLerman L Set al.Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis[J].Nucleic Acids Research198513(9)3131-3145.

[11]Myers R MFischer S GManiatis Tet al.Modification of the melting properties of duplex DNA by attachment of a GC-rich DNA sequence as determined by denaturing gradient gel electrophoresis[J].Nucleic Acids Research198513(9)3111-3129.

[12]Fantroussi SVerschuere LVerstraete Wet al.Effect of phenylurea herbieides on soil microbial communities estimated by analysis of rRNA gene fingerprints and community-level physiological profiles[J].Applied and Environmental Microbiology199965982-988.

[13]贾建丽,李广贺,张旭,等.基于PCR-DGGE技术的石油污染土壤微生物多态性[J].清华大学学报(自然科学版)200591217-1220.

[14]胡元森,吴坤,李翠香,等.黄瓜连作对土壤微生物区系影响II—基于DGGE方法对微生物种群的变化分析[J].中国农业科学,200740(10)2267-2273.

[15]孙晓棠,王燕,龙良鲲.番茄根际微生物种群动态变化及多样性[J].微生物学通报,200835(11):1744 -1749.

[16]王岳坤,洪葵.红树林土壤细菌群落16S rDNA V3片段PCR 的产物DGGE分析[J].微生物学报,200545(2):201-204.

[17]Allison EMccaing LAnne Get al.Numerical Analysis of Grassland Bacterial Community Structure under Different Land Management Regimens by Using 16S Ribosomal DNA Sequence Data and Denaturing Gradient Gel Electrophoresis Banding Patterns [J].Applied and Environmental Microbiology200167(10)4554-4559.

[18]Ferris M JMuyzer GWard D M.Denaturing gradient gel electrophoresis profiles of 16S rRNA defined populations inhabiting a hotspring microbial mat community[J].Appl Environ Microbiol199662340-346.


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备注/Memo

基金项目:黑龙江省科技厅国际合作重点资助项目(WB07A10)

第一作者简介:宋洋(1981-,女,黑龙江省哈尔滨市人,硕士,研究实习员,主要从事微生物菌群分析和分子育种研究。E-mail:achievement81@yahoo.com.cn。
通讯作者:李柱刚(1972-,男,黑龙江省庆安县人,博士,研究员,从事植物分子育种研究。E-mail:lizhugang@163.com。

更新日期/Last Update: 2014-11-03