XU Mei-long,QIN Bin-bin,Jiang Bai -yang,et al.Establishment of a Transgenic Acceptor System for Solanum Tuberosum L .[J].HEILONGJIANG AGRICULTURAL SCIENCES,2009,(06):1-3,8.[doi:10.11942/j.isn1002-2767.2009.06.0001]
马铃薯转基因受体系统的建立
- Title:
- Establishment of a Transgenic Acceptor System for Solanum Tuberosum L .
- 文章编号:
- 1002-2767(2009)06-0001-03
- Keywords:
- Key words:Solanumtuberosum L.; transgene; receptor system
- 分类号:
- S532
- 文献标志码:
- A
- 摘要:
- 摘要:以马铃薯(Solanumtuberosum L.)“Wen-40”和“紫花白”2个品种的茎段和叶片为外植体, 通过离体培养分化成苗以及对卡那霉素(Km)和头孢霉素(Cef)的敏感性研究, 建立了农杆茵介导的稳定、高效的基因转化受体系统。结果表明:“Wen-40”的茎段适合转基因高效再生体系的建立, 而 25 mg ·L -1 的 Km 适合转基因植株的抗性筛选, 低于200 mg·L -1 的 Cef浓度对马铃薯的诱导分化影响较小。
- Abstract:
- Abstract:Stem segments and blades were used as explants of tissue culture of Solanum tuberosum L.varieties“Wen-40”and“Zihuabai”, plantlet regeneration and effects of antibiotics, including kanamycin(Km), and cefotaxime(Cef), on the inductionof callus were studied.The results indicated that stem segments was more suitable for establishment of regeneration systemthan blades.The optimized concentration of kanamycin for selecting the stem segments was 25 mg·L -1 and cefotaxime wasless than 200 mg·L -1 .
参考文献/References:
[ 1] 齐恩芳, 张金文, 王一航.马铃薯茎段再生的植物激素配比优化[ J] .甘肃农业大学学报, 2006(6):14-17.
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备注/Memo
第一作者简介:徐美隆(1979-), 男, 四川省大竹县人, 硕士,助理研究员, 主要从事植物组织培养研究。E-mail:xml1106007@163.com