[1]李德军,邓　 治,陈春柳,等.巴西橡胶树核糖体蛋白S6基因的克隆及生物信息学分析[J].黑龙江农业科学,2009,(06):4-8.[doi:10.11942/j.isn1002-2767.2009.06.0004]
　LI De-jun,DENGZhi,CHEN Chun-liu,et al.Cloning and Bioinformatic Analysis of the Gene Encoding the RibosomalProtein S6 from Hevea brasiliensis[J].HEILONGJIANG AGRICULTURAL SCIENCES,2009,(06):4-8.[doi:10.11942/j.isn1002-2767.2009.06.0004]
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巴西橡胶树核糖体蛋白S6基因的克隆及生物信息学分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2009年06 页码: 4-8 栏目: 出版日期: 2009-11-10
Title:
Cloning and Bioinformatic Analysis of the Gene Encoding the RibosomalProtein S6 from Hevea brasiliensis
文章编号:
1002-2767(2009)06-0004-05
作者:
李德军; 邓　 治; 陈春柳; 陈守才
(中国热带农业科学院 橡胶研究所/农业部橡胶生物学重点开放实验室,海南 儋州 571737)
Author(s):
LI De-jun; DENGZhi ; CHEN Chun-liu; CHEN Shou-cai
(Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Rub-ber Biology,Ministry of Agriculture,Danzhou,Hainan 571737)
关键词:
关键词:巴西橡胶树; 核糖体蛋白 S6; 基因克隆; 序列分析
Keywords:
Key words:Heveabrasiliensis; ribosomal protein S6; gene cloning; sequence analysis
分类号:
S794.1
DOI:
10.11942/j.isn1002-2767.2009.06.0004
文献标志码:
A
摘要:
摘要:核糖体蛋白S6是核糖体蛋白家族的重要成员, 在核糖体中发挥重要作用。利用农业部橡胶生物学重点开放实验室获得的部分序列设计引物, 采用 3′-RACE技术从巴西橡胶树胶乳中获得一个核糖体蛋白 S6 基因的完整阅读框,该基因编码 249 个氨基酸的多肽, 含有一个典型的真核生物核糖体蛋白 S6 结构域。在 HbRPS6 氨基酸序列中没有预测到跨膜区和信号肽, 二级结构分析表明:HbRPS6属于混合型蛋白。 对10个 RPS6系统进化分析表明:巴西橡胶树的RPS6基因在进化上具有保守性, 与植物类玉米的 RPS5 基因亲缘关系最近, 而与动物和酵母的亲缘关系相对较远。该基因的克隆和生物信息学分析为深入研究其功能奠定了基础。
Abstract:
Abstract:The ribosomal protein S6 is the key member of ribosomalprotein family, and it plays an important role in the ribo-somal proteins.The primers designed based on the known sequences acquired from Key Laboratory of Rubber Bidoye ofMinisby of Agriculture, the gene wascloned with 3′-RACE technique from Heveabrasiliensis latex.The open reading frameencoded 249 amino acids, containing a conserved ribosomal S6 domain, so the gene was namedas HbRPS6.No signal peptideand transmembrane structure were predicted within HbRPS6.The result of secondary structure predicted showed that theprotein was classified as mixed type.The result of phylogenetic tree indicated that HbRPS6 displayed a high degree of se-quence similarity to ZmRPS5, but not to animals and yeast.This paper will be benefit to further analyze the functions ofHbRPS6.

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备注/Memo

基金项目:国家天然橡胶现代农业产业技术体系建设项目(nycytx-34-GW1-2);中国热带农业科学院橡胶研究所基本科研业务费专项资金资助项目(XJSYWFZX2009-08)第一作者简介:李德军(1975-), 男, 辽宁朝阳人, 博士, 助理研究员,从事巴西橡胶树死皮病分子机制与防治研究。E-mail:djli.rricatas@gmail.com。

更新日期/Last Update: 2016-11-28