[1]张冬杰.维生素A 对视黄素X 受体β 基因的诱导表达研究[J].黑龙江农业科学,2009,(03):1-2.[doi:10.11942/j.isn1002-2767.2009.03.0001]
　ZHANGDong-jie.Study of Vitamin A on the Expression of Retinoid X Receptor β Gene[J].HEILONGJIANG AGRICULTURAL SCIENCES,2009,(03):1-2.[doi:10.11942/j.isn1002-2767.2009.03.0001]
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维生素A 对视黄素X 受体β 基因的诱导表达研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2009年03 页码: 1-2 栏目: 出版日期: 2009-05-10
Title:
Study of Vitamin A on the Expression of Retinoid X Receptor β Gene
文章编号:
1002-2767(2009)03-0001-02
作者:
张冬杰
(黑龙江省农业科学院畜牧研究所, 黑龙江哈尔滨150086)
Author(s):
ZHANGDong-jie
(Animal Husbandry Research Institute of Heilongjiang Academy of Agricultural Sciences ,Harbin ,Heilongjiang150086)
关键词:
猪; 视黄酸受体β 基因; 成纤维细胞; RT-PCR
Keywords:
pig ; RXRβ ; fibroblast cells; RT-PCR
分类号:
S858
DOI:
10.11942/j.isn1002-2767.2009.03.0001
文献标志码:
A
摘要:
采用RT-PCR技术检测分析了不同浓度的维生素A 在猪的成纤维细胞内分别作用12、24 和48 h 后, 视黄素X 受体β 基因(RXRβ)的表达变化情况。结果表明:不同浓度的维生素A 对RXRβ 基因的作用效果不同, 12 h 时, 30.0 μg ·μL-1组可显著诱导RXRβ 基因的表达,24 h 时, 10 .0 μg·μL-1 组可显著诱导RXRβ 基因的表达, 48 h 时, 各组与对照组之间不存在差异。
Abstract:
In the present study , RXRβ ex pression level was detected by RT-PCR in fibroblast cells cultured in different concentrationvitamin A media and cultured 12, 24, 48 h, respectively .The results showed that different concentration of vitaminA had different induced effect.30.0 μg·μL-1 group could sig nificantly induce RXRβ expression on 12 h, but 10.0 μg ·μL-1group w as on 24 h, every g roup had no difference compared with the control g roup on 48 h .

参考文献/References:

[ 1] 　Man gels dorf D J , Ong E S , Dyck J A , et al.Nucl ear recepto rth at iden tif ies a novel retinoi c acid respon se pathw ay[ J] .Nature , 1990 , 345(6272):224-229 .

[ 2] 　Flei schh auer K, McBride O W , DiS an to J P , et al.Cloning andchromos om e mapping of human ret inoid X recep tor b et a:selectiveamino acid sequence conservat ion of a nuclear h ormone receptor in mam mal s[ J] .H um Genet , 1993 , 90(5):505-510 .
[ 3] 　Fi tz gibbon J , Gi llet t G T , Woodw ard K J , et al.Mapping ofRXRB t o human ch romosome 6p21 .3[ J] .An n H um Genet ,1993, 57(3):203-209 .

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备注/Memo

作者简介:张冬杰(1980-), 女, 黑龙江省佳木斯市人, 博士,助理研究员, 主要从事猪的分子遗传育种研究。Tel :13845091493 ;E-mail:djzhang8109 @163 .com 。

更新日期/Last Update: 2016-09-08