SHAO Ju-fang,ZHU Hong-wei,ZHANG Zhi,et al.Effects of Several Factors on Hairy Root Inducement andFlavone Content of Ginkgo biloba[J].HEILONGJIANG AGRICULTURAL SCIENCES,2008,(06):10-12.[doi:10.11942/j.issn1002-2767.2008.06.0010]
几种因素对银杏毛状根诱导和黄酮含量的影响
- Title:
- Effects of Several Factors on Hairy Root Inducement andFlavone Content of Ginkgo biloba
- 文章编号:
- 1002-2767(2008)06-0010-03
- Keywords:
- factor ; Gink go bi loba ; hairy ro ot ; inducement
- 分类号:
- S792 .95 .08
- 文献标志码:
- A
- 摘要:
- 考察了发根农杆菌菌种、侵染时间、外植体类型、培养基类型和乙酰丁香酮5 个因素对银杏毛状根诱导的影响, 以及比较了后二因素对毛状根黄酮积累的影响。结果表明:以银杏胚为外植体, A4 活化菌液中加入乙酰丁香酮100 μmo l·L-1 侵染15 min , 再在MS 培养基上培养发根率最高。乙酰丁香酮对毛状根中黄酮的积累没有显著促进作用, 浓度高于120 μmo l·L-1 还会严重抑制发根。
- Abstract:
- Five facto rs which influence inducement o f Gink go biloba hair y ro ot s, including Ag ro bacterium rhizogenesty pe s, intrusion time , ex plant ty pe s, medium ty pes and AS wer e rev iew ed in this ar ticle .The effect of thetwo later facto rs o n flav ones content in hairy roo ts w as also compa red.The r esults showed tha t :Ro oting rates w asthe highest in the track tha t embry o intruded by activity A4 w ith 100 mol·L-1 AS fo r 15 minutes and then culturedon M S medium .There was no mar kedly simulative functio n of AS fo r flavo ne accumulatio n in hairy ro o ts.Atthe same time, ro oting ra te w as badly restr ained w hen AS co ntent w as higher than 120 mo l·L -1 .
参考文献/References:
[ 1] 庄向平, 虞杏英, 杨更生, 等.银杏叶中黄酮含量的测定和提取方法[ J] .中草药, 1992 , 23(3):122-124 .
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备注/Memo
基金项目:中国矿业大学科技基金项目“ 银杏转基因发根培养研究” (OH061009 );“ 高产银杏黄酮悬浮细胞系的建立(OH 060101);中国矿业大学大学生科研训练计划项目(070604)