[1]郭伟珍,林艳,曹军合,等.美洲南蛇藤组织培养技术[J].黑龙江农业科学,2008,(05):19-21.[doi:10.11942/j.issn1002-2767.2008.05.0019]
　GUO Wei-zhen,LIN Yan,CAO Jun-he,et al.Technique of Tissue Culture on Celart rus scandens[J].HEILONGJIANG AGRICULTURAL SCIENCES,2008,(05):19-21.[doi:10.11942/j.issn1002-2767.2008.05.0019]
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美洲南蛇藤组织培养技术

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2008年05 页码: 19-21 栏目: 出版日期: 2008-09-10
Title:
Technique of Tissue Culture on Celart rus scandens
文章编号:
1002-2767(2008)05-0019-03
作者:
郭伟珍1 ;  林艳1 ;  曹军合2 ;  邢存旺1
1 .河北省林业科学研究院, 石家庄050061 ;
2 .新乐市林业局, 新乐050700
Author(s):
GUO Wei-zhen1 ;  LIN Yan1 ; CAO Jun-he2 ; XING Cun-wang1
1 .Hebei Academy of Fo rest ry Sciences , Shijiazhuang 050061 ;
2 .Xinle City Forestry Bureau ,Xinle 050700
关键词:
美洲南蛇藤; 生长调节剂; 继代增殖; 过渡移栽
Keywords:
Celartrus scandens ; plant g rowth reg ulato r ; subculture multiplication ; transitio n cultivation
分类号:
S687 .3
DOI:
10.11942/j.issn1002-2767.2008.05.0019
文献标志码:
A
摘要:
以美洲南蛇藤幼嫩的顶芽或带腋芽的茎段为外植体, 进行外植体消毒、增殖培养、瓶内生根和过渡移栽研究, 结果表明:外植体用75%的乙醇消毒20 s , 0 .1 %的HgCl2 消毒2.0 min 效果最佳;适宜的增殖培养基为M S+6-BA 1 .0mg ·L-1(单位下同)+IBA 0 .2 +3.0 %蔗糖;壮苗培养基为MS +6-BA 0.1 + IBA 0 .05 +3%蔗糖;适宜的生根培养基为1/ 2MS +IBA 0.5+1 .5 %蔗糖, 生根率82 .0 %;最佳过渡移栽基质为蛭石∶珍珠岩=2∶1, 成活率为98 .3 %。
Abstract:
Taking top buds and stems with axillary buds as the e xplants , the study of disinfection , pro life ratio n culture, r oo ting in vitro and t ransition cultiva ting were conducted.The results w ere as fo llow s:the most suitable disinfecttime pr operly wa s 20 s by alcohol and 2 min by HgCl2 respectively ;pro liferatio n culture media wa s MS +6-BA1.0mg ·L-1 +IBA0.2 +Sucr ose 3 %;ha rde ning-off culture media wa s MS +6-BA0.1 + IBA0.05 +Sucro se3%;r oo ting cultur e media w as 1/ 2MS +IBA 0 .5 + Sucro se 1.5% and its r oo ting ratio w as 82.0%;transitio n cultivating media was pearlite and peat , its rate wa s 2 ∶1, sur viv e ratio wa s 98.3%.

参考文献/References:

[ 1] 郭远强, 李铣.南蛇藤属植物化学成分研究进展[ J] .沈阳药科大学学报, 2003 , 20(3):226-229 .

[ 2] 徐振华.美洲南蛇藤优良资源及栽培技术引进在冀实施[ J] .林业科技开发, 2003 , 17(5):76 .

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备注/Memo

基金项目:“ 948” 国际先进农业科学技术引进项目(2003-4-22)第一作者简介:郭伟珍(1971-),女, 河北新乐人, 学士, 林业高级工程师, 从事良种繁育研究。E-mai l:gw zcjh @yahoo .com .cn 。

更新日期/Last Update: 2016-12-16