[1]辛玉峰,曲晓华.一株石油降解菌的筛选、分离纯化及降解性能分析[J].黑龙江农业科学,2008,(01):17-18.[doi:10.11942/j.issn1002-2767.2008.01.0017]
　XIN Yu-feng,QU Xiao-hua.Screen ,Isolation and Analysis of Degradation Capabilityfor a Crude Oil Degrading Strains[J].HEILONGJIANG AGRICULTURAL SCIENCES,2008,(01):17-18.[doi:10.11942/j.issn1002-2767.2008.01.0017]
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一株石油降解菌的筛选、分离纯化及降解性能分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2008年01 页码: 17-18 栏目: 出版日期: 2008-01-10
Title:
Screen ,Isolation and Analysis of Degradation Capabilityfor a Crude Oil Degrading Strains
文章编号:
1002-2767(2008)01-0017-02
作者:
辛玉峰;  曲晓华
曲阜师范大学生命科学学院, 曲阜273165
Author(s):
XIN Yu-feng ; QU Xiao-hua
Life Science College ,Qufu Normal University ,Qufu 273165
关键词:
石油降解菌; 分离; 纯化
Keywords:
crude oil deg rading strain; isolatio n; purification
分类号:
Q93-331
DOI:
10.11942/j.issn1002-2767.2008.01.0017
文献标志码:
A
摘要:
从胜利油田污染土壤中分离纯化得到石油降解菌, 并对其部分性状及生理生化特性进行了实验研究。经鉴定该菌株属于假单胞菌属, 能够以石油作为唯一碳源生长。通过光谱扫描200 ～ 600 nm 范围内石油的吸光度确定在211 nm 处石油有较高吸收峰。通过测量不同时间段菌的悬浮液在600 nm 和211 nm 处的吸光度, 结果表明随着菌的生长211 nm 处的吸收峰显著下降。这表明该菌能以石油为碳源且降解能力较强。
Abstract:
A strain o f oil deg rading w as separa ted and purified from the polluted soil o f Shengli Oil Field.Its sometraits and phy siological bio chemistr y characteristic we re studied .The strain w as pseudomonas sp.It co uld g row nby the only pe troleum .With SP -756P spect rophotome te r scanned in 200 ～ 600 nm sco pe , thro ug h the spec trumde te rminatio n of the petro leum ex tinctio n.The petro leum absor ption peak w as at 211 nm place .Thro ug h measuredthe e xtinctio n o f suspe nding liquid of fungus in 600 nm and 211 nm at the different time , the result show ed tha tthe abso rption peak o f 211 nm remarkable dro pped alo ng w ith the g row th o f the st rain .This indicated that thisstrain could take pe troleum a s carbon source and the deg radatio n capability w as strong

参考文献/References:

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[ 2] Vecchioli G I , Del Panno M T , Paincei ra M T .U se of select edau t ochthonous s oi l bact eri a t o enhance deg radati on of hyd rocarbonsin s oi l [ J] .Environment al Pollut ion , 1990 , 67 (3):24-258 .
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备注/Memo

基金项目:曲阜师范大学科研启动基金资金

第一作者简介:辛玉峰(1981-), 男, 山东省潍坊市人, 硕士, 讲师, 从事环境微生物学研究。E-mai l:xinyuf en g520 @163 .com 。

更新日期/Last Update: 2016-12-12