[1]李国良.热导系玉米种子贮存蛋白的生物指纹图谱分析[J].黑龙江农业科学,2005,(06):25-27.[doi:10.11942/j.issn1002-2767.2005.06.0025]
　LI Guo-liang.Finger Print Analysis on Seed Protein of Maize Inbred Line[J].HEILONGJIANG AGRICULTURAL SCIENCES,2005,(06):25-27.[doi:10.11942/j.issn1002-2767.2005.06.0025]
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热导系玉米种子贮存蛋白的生物指纹图谱分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2005年06 页码: 25-27 栏目: 出版日期: 2005-11-10
Title:
Finger Print Analysis on Seed Protein of Maize Inbred Line
文章编号:
1002-2767(2005)06-0025-03
作者:
李国良
Author(s):
LI Guo-liang
Maize Research Institute of Heilongjiang Academy of Agricultural Sciences,Harbin 150086
关键词:
玉米; 聚丙稀酰胺凝胶电泳; 指纹图谱
Keywords:
maize;  SDS-PAGE;  finger print
分类号:
S513
DOI:
10.11942/j.issn1002-2767.2005.06.0025
文献标志码:
A
摘要:
利用聚丙稀酰胺凝胶电泳(SDS-PAGE)对热导系玉米种子的可溶性蛋白进行分析，结果表 明，清蛋白、球蛋白、谷蛋白都显示出丰富的带谱。自交系间谱带数目、带的强弱与分布等特点均可 见较明显的差异，可将各供试自交系互相区分出来；醇溶蛋白谱带均较弱，自交系间差异小，难以把 各供试自交系互相区分出来。尽管种子的可溶蛋白质 PAGE 图谱都可作为玉米自交系鉴定的蛋 白质指纹图谱，相比之下，利用清蛋白 PAGE 图谱较为理想。
Abstract:
Finger Print Analysis on Seed Protein of Maize Inbred Line1002-2767(2005)06-0025-03Abstract: Electrophoretic analysis was made on the seed soluble protein of 9 maize inbred lines bypolyacryamide gel electrophoresis (PAGE) techniques . The results showed that the zeins wereboth simple and differences among varieties were small. It was ,therefore, difficult to distinguishall the varieties studied with the zein patterns, Though, the PAGE of the seed soluble proteins allcan be regarded as protein fingerprint for identification of 9 maize varieties, the PAGE patterns ofthe albumins were ,however, abundarit and the differences among varieties were obvious in bandsnumber distribution and concentration . On the basis of these characteristics ,all the varietiesstudied could be discriminated from each other .It was suggested that PAGE of seed albumin wasuseful in variety identification in maize.

参考文献/References:

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备注/Memo

收稿日期：2005-07-14

作者简介：李国良（1973-），男，黑龙江省肇东市人，助理研究员，从事玉米育种研究。

更新日期/Last Update: 2016-12-09