ZHANG A-ying,HU Bao-zhong,JIANG Shu-jun,et al.Establishment of Reaction System for the Assay and Usage of SSR in Alfalfa[J].HEILONGJIANG AGRICULTURAL SCIENCES,2002,(01):15-17.[doi:10.11942/j.issn1002-2767.2002.01.0015]
影响紫花苜蓿SSR 分析因素的研究
- Title:
- Establishment of Reaction System for the Assay and Usage of SSR in Alfalfa
- 文章编号:
- 1002 -2767(2002)01 -0015 -03
- Keywords:
- alfalfa ; simple sequence repeat ; affecting factors
- 分类号:
- S551 .7
- 文献标志码:
- A
- 摘要:
- 以我国紫花苜蓿地方品种为研究材料, 利用1 对微卫星引物进行SSR 扩增反应体系探讨, 确立了最佳SSR 反应体系。
- Abstract:
- Local samples in Medicago sativa were studied using SSR technique .Based on the genomic DNAextracted from alfalfa , essential factors affecting the result of SSR assay were tested and compared .An optimalreaction system suitable for the assay and usage of SSR in alfalfa was established .
参考文献/References:
[1] Akkaya M S , Bhagwat A .A ,Cregan P .B.Length polymorphisms of simplesequence repeat DNA in Soybean[ J] .Geneti cs , 1992 , 132 :1131-1139.
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备注/Memo
收稿日期:2001 -10-03