YANG Meng-ping.Study on Biological Characteristics of Pathogen Causing Potato Scab in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(05):45-49.[doi:10.11942/j.issn1002-2767.2022.05.0045]
黑龙江省马铃薯疮痂病致病菌生物学特性研究
- Title:
- Study on Biological Characteristics of Pathogen Causing Potato Scab in Heilongjiang Province
- 文献标志码:
- A
- 摘要:
- 为研究黑龙江省马铃薯疮痂病菌的生物学特性，对黑龙江省3种马铃薯疮痂病致病菌Streptomyces scabies、Streptomyces turgidiscabies、Streptomyces acidiscabies进行温度、pH、盐耐受性、碳氮源利用以及抗生素敏感性试验。结果表明：供试菌株最适生长温度均为29 ℃，生长所需pH范围及生长最适pH存在较大差异，S.scabies可生长pH范围为5.0~10.0，最适生长pH为8.0；S.turgidiscabies可生长pH范围为6.0~10.0，最适pH为9.0；S.acidiscabies可生长pH范围为4.0~8.0，最适pH为6.0；盐耐受浓度无差异，均低于4%；生长可利用碳源无差异，均能以葡萄糖、蔗糖、果糖、木糖、甘露醇、肌醇、乳糖、鼠李糖、阿拉伯糖作为单一碳源；可利用氮源差异较大，S.scabies可以酪氨酸、半胱氨酸、甲硫氨酸、甘氨酸、丙氨酸为单一氮源；S.turgidiscabies可以酪氨酸、脯氨酸、半胱氨酸、甘氨酸、丙氨酸为单一氮源；S.acidiscabies可以酪氨酸和丙氨酸为单一氮源；对不同种抗生素的敏感性也存在差异，S.scabies对青霉素G、赤霉素、卡那霉素敏感；S.turgidiscabies对青霉素G、多粘菌素B、赤霉素、卡那霉素、硫酸盐链霉素、硫酸盐庆大霉素、氯霉素敏感；S.acidiscabies对青霉素G、赤霉素、硫酸盐链霉素、氯霉素敏感。综上所述，黑龙江地区马铃薯疮痂病致病菌的生物学特性存在较大的种间差距。
- Abstract:
- To study the biological characteristics of potato scab pathogen in Heilongjiang Province,Streptomyces scabies,Streptomyces turgidiscabies and Streptomyces acidiscabies the three pathogenic bacteria of potato scab in Heilongjiang Province were taken as research materials.We studied the temperature,pH,salt tolerance tests,carbon,nitrogen source utilization tests and antibiotic sensitivity tests.The results showed that the optimum growth temperature of the tested strains was 29 ℃,pH range of S.scabies was 5.0-10.0,and the optimum pH was 8.0.S.turgidiscabies grew in the range of pH6.0 to 10.0,and the optimal growth pH was 9.0.The growth range of S.acidiscabies was pH4.0 to 8.0,and the optimum growth pH was 6.0.There was no difference in salt tolerance concentration,which was lower than 4%.Glucose,sucrose,fructose,xylose,mannitol,inositol,lactose,rhamnose and arabinose could use as a single carbon source for the normal growth of potato scab.The available nitrogen sources of different strains were quite different,And S.scabies could use tyrosine,cysteine,methionine,glycine and alanine as a single nitrogen sources.S.turgidiscabies can take tyrosine,proline,cysteine,glycine and alanine as a single nitrogen source.S.acidiscabies can use tyrosine and alanine as a single nitrogen source.S.scabies was sensitive to penicillin G,gibberellin and kanamycin.S.turgidiscabies were sensitive to penicillin G,polymyxin B,gibberellin,kanamycin,streptomycin sulfate,and gentamicin sulfate,and chloramphenicol; S.acidiscabies were sensitive to penicillin G,gibberellin,streptomycin sulfate and chloramphenicol.In conclusion,there are great interspecific differences in the biological characteristics of potato scab pathogen in Heilongjiang Province.
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相似文献/References:
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备注/Memo
收稿日期：2022-01-14
基金项目：科技部、财政部、国家科技资源共享服务平台项目“国家作物种质资源库马铃薯分库运行服务”（NCGRC-2021-44)；农业农村部物种保护项目“马铃薯种质资源的收集、鉴定、编目、繁种与入库（圃）保存”（19210136）。
作者简介：杨梦平（1991-），女，硕士，研究实习员，从事马铃薯种质资源保存与利用研究。E-mail：396162469@qq.com。