[1]房海悦.旱半夏组织培养体系优化[J].黑龙江农业科学,2019,(09):28-32.[doi:10.11942/j.issn1002-2767.2019.09.0028]
　FANG Hai-yue.Optimization on Tissue Culture System of Pinellia ternata[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(09):28-32.[doi:10.11942/j.issn1002-2767.2019.09.0028]
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旱半夏组织培养体系优化

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2019年09期 页码: 28-32 栏目: 出版日期: 2019-09-10
Title:
Optimization on Tissue Culture System of Pinellia ternata
作者:
房海悦
1.拜泉县产业项目服务中心，黑龙江 拜泉 164700；2.齐齐哈尔市北方本草经济植物研究所，黑龙江 拜泉 164700
Author(s):
FANG Hai-yue
1.Baiquan Industrial Project Service Center,Baiquan 164700,China;2.Qiqihar Northern Herbal Economic Plants Research Institute,Baiquan 161000，China
关键词:
半夏; 愈伤组织; 分化; 诱导
Keywords:
Pinellia ternate;  callus;  differentiation;  induce
DOI:
10.11942/j.issn1002-2767.2019.09.0028
文献标志码:
A
摘要:
为研究旱半夏组织培养体系的最优激素配比，本试验以旱半夏块茎为材料,以MS为基础培养基，添加不同浓度的6-BA、NAA激素，设置9个处理，筛选诱导愈伤组织、不定芽分化以及生根最适培养基。研究了不同激素以及不同的浓度配比对半夏组织培养的影响。结果表明：6-BA、NAA两种激素以及不同浓度配比均对半夏愈伤组织诱导、不定芽分化以及生根有显著影响，其中添加0.8 mg·L-1-6-BA和0.4 mg·L-1-NAA时出芽数最多，出芽率达到90%；生根培养中，添加1.0 mg·L-1-NAA时生根数最多，平均根长达到6.4 cm。因此，半夏最佳愈伤组织诱导以及不定芽分化的条件为：MS+0.8 mg·L-1- 6-BA+0.4 mg·L-1-NAA；最佳生根培养基为：1/2 MS+1.0 mg·L -1-NAA。
Abstract:
In order to study the optimal hormone ratio of tissue culture system of Pinellia ternata,the experiment used tuber of Pinellia ternata as material,MS as base medium,6-BA and NAA hormones of different concentration were added,and 9 treatments were set up to screen the best medium for inducing callus,adventitious bud differentiation and rooting.The effects of different hormones and concentrations on tissue culture of Pinellia ternata were studied.The results showed that both 6-BA and NAA hormones and their ratios at different concentrations had significant effects on callus induction,adventitious bud differentiation and rooting of Pinellia ternata.Among them,0.8 mg·L-1- 6-BA and 0.4 mg·L-1- NAA had the highest number of buds and 90% of buds,and 1.0 mg·L-1-NAA had the highest number of roots and the average length of roots in rooting culture.Therefore,the optimum conditions for callus induction and adventitious bud differentiation of Pinellia ternata were MS+0.8 mg·L-1-6-BA+0.4 mg·L-1- NAA,and the optimum rooting medium was 1/2 MS+1.0 mg·L-1--NAA.

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备注/Memo

收稿日期：2019-03-26

作者简介：房海悦（1991-），女，硕士，从事植物组织培养研究。E-mail:fanghaiyue1991@126.com。

更新日期/Last Update: 2019-09-17