[1]张淑红,吕聪真,石林成,等.枝干番杏组织培养技术[J].黑龙江农业科学,2019,(03):21-24.[doi:10.11942/j.issn1002-2767.2019.03.0021]
　ZHANG Shu-hong,LYU Cong-zhen,SHI Lin-cheng,et al.Techniques of Tissue Culture with Dicrocaulon ramulosum(L.Bolus) Ihlenf[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(03):21-24.[doi:10.11942/j.issn1002-2767.2019.03.0021]
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枝干番杏组织培养技术

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2019年03期 页码: 21-24 栏目: 出版日期: 2019-03-10
Title:
Techniques of Tissue Culture with Dicrocaulon ramulosum(L.Bolus) Ihlenf
作者:
张淑红;  吕聪真;  石林成;  高金红; 贾方方;  马丽
商丘师范学院 生物与食品学院， 河南 商丘 476000
Author(s):
ZHANG Shu-hong; LYU Cong-zhen; SHI Lin-cheng; GAO Jin-hong; JIA Fang-fang; MA Li
College of Biology and Food,Shangqiu Normal University,Shangqiu 476000,China
关键词:
枝干番杏; 组织培养; 带叶茎段; 芽诱导; 根诱导
Keywords:
Dicrocaulon ramulosum(L.Bolus) Ihlenf;  tissue culture;  stem;  bud induction;  root induction
DOI:
10.11942/j.issn1002-2767.2019.03.0021
文献标志码:
A
摘要:
以枝干番杏的带叶茎段为外植体，比较不同灭菌方法的灭菌效果，及不同激素浓度对丛生芽诱导及生根的影响。结果表明：本研究中枝干番杏组织培养不经过愈伤组织阶段，芽诱导最适宜的培养基为MS+6-BA 8.325 mg?L-1+NAA 16.65 mg?L-1。芽丛生继代最适培养基为MS+6-BA 2.4 mg?L-1+NAA 9.6 mg?L-1，根诱导最适培养基为1/2 MS+6-BA 1.2 mg?L-1+NAA 4.8 mg?L-1。枝干番杏的离体快繁体系有望用在番杏科其他植物的组培中。
Abstract:
The leaf stems of Dicrocaulon ramulosum(L.Bolus) Ihlenf were used as explants to compare the effects of different sterilization methods,as well as the effects of different hormone concentrations on the induction of shoots and roots.The results showed that the tissue culture did not pass through the callus stage.The optimal medium for shoot induction,bud subculture and root induction was MS+6-BA 8.325 mg?L-1+NAA 16.65 mg?L-1,MS+6-BA 2.4 mg?L-1+NAA 9.6 mg?L-1,and 1/2 MS+6-BA 1.2 mg?L-1+NAA 4.8 mg?L-1,respectively.The in vitro rapid propagation system of Dicrocaulon ramulosum(L.Bolus) Ihlenf was expected to be used in the tissue culture of other plants within the Aizoaceae family.

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备注/Memo

收稿日期：2018-09-28

基金项目：河南省科技厅科技攻关项目（162102110092，182102110130）。
第一作者简介：张淑红（1977-），女，博士，教授，从事植物组织培养教学工作。E-mail：shuhongzhang_2013@163.com。

更新日期/Last Update: 2019-03-24