[1]李贤,张爱冬,朱宗文,等.嘉定白蒜的病毒检测[J].黑龙江农业科学,2018,(07):35-38.[doi:10.11942/j.issn1002-2767.2018.07.0035]
　LI Xian,ZHANG Ai-dong,ZHU Zong-wen,et al.Viruses Detection of Jiading White Garlic[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(07):35-38.[doi:10.11942/j.issn1002-2767.2018.07.0035]
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嘉定白蒜的病毒检测

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2018年07期 页码: 35-38 栏目: 出版日期: 2018-07-10
Title:
Viruses Detection of Jiading White Garlic
作者:
李贤1; 张爱冬1; 朱宗文1; 吴雪霞1; 查丁石1; 高畅2
1.上海市农业科学院 园艺研究所,上海市设施园艺技术重点实验室,上海 201403；2.上海蔬菜研究所,上海 201899
Author(s):
LI Xian1; ZHANG Ai-dong1; ZHU Zong-wen1; WU Xue-xia1; ZHA Ding-shi1; GAO Chang2
1.Horticultural Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai Key Laboratory of Protected Horticultural Technology,Shanghai 201403,China; 2.Shanghai Research Institute of Vegetable,Shanghai 201899,China
关键词:
嘉定白蒜; RT-PCR; 大蒜普通潜隐病毒; 洋葱黄矮病毒
Keywords:
Allium sativum L.;  RT-PCR;  garlic common latent virus(GCLV);  onion yellow dwarf virus(OYDV)
DOI:
10.11942/j.issn1002-2767.2018.07.0035
文献标志码:
A
摘要:
为明确嘉定白蒜在种植区的病毒情况，从嘉定白蒜两个种植基地采样，分别提取总RNA，然后反转录。采用RT-PCR方法检测洋葱黄矮病毒（OYDV）和大蒜普通潜隐病毒（GCLV）。结果表明：根据GCLV中间保守序列设计3对特异性引物，在两处样品中均分别扩增到300、710和450 bp片断。将其中450 bp片断克隆后测序。测序结果与GenBank上的GCLV基因序列比对，发现其同源性达到95%。由此确认嘉定白蒜中含有GCLV。根据OYDV中间保守序列设计5对引物，在两处样品中均未扩增到可检测的片断，表明嘉定白蒜中不含OYDV。
Abstract:
In order to clarify the virus situation in Jiading white garlic,total RNA was extracted from samples,which collected from two Jiading white garlic planting bases,and cDNA was synthesized using reverse transcriptase.Onion yellow dwarf virus(OYDV) and garlic common latent virus(GCLV) were detected by RT-PCR.The results showed that 3 pairs of specific primers were designed according to the GCLV intermediate conservative sequence.We amplified and got 300,710 and 450 bp fragments from two samples respectively.We cloned the 450 bp fragment and sequenced.The sequencing result was compared with GCLV gene sequence in GenBank and the homology rate reached 95%,which confirmed the existence of GCLV in Jiading white garlic.According to conserved sequence of OYDV,we designed 5 pairs of specific primers.However,we didn’t obtain the amplified fragments,indicating Jiading white garlic in two planting bases contained no OYDV.

参考文献/References:

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备注/Memo

收稿日期：2018-02-08基金项目：上海市种业发展资助项目(沪农科种字2016第1-11号）。第一作者简介：李贤（1962-），男，博士，副研究员，从事蔬菜生物技术研究。E-mail:Xianlisiwei@163.com。通讯作者：查丁石（1966-），男，博士，研究员，从事蔬菜育种及生物技术研究。E-mail:dingshizha@aliyun.com。

更新日期/Last Update: 2018-10-31