[1]秦瑶,董书言,高婷,等.凤丹白牡丹花粉活力测定[J].黑龙江农业科学,2018,(04):96-98.[doi:10.11942/j.issn1002-2767.2018.04.0096]
　QIN Yao,DONG Shu-yan,GAO Ting,et al.Determination of Pollen Viability of Paeonia ostii Fengdanbai[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(04):96-98.[doi:10.11942/j.issn1002-2767.2018.04.0096]
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凤丹白牡丹花粉活力测定

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2018年04期 页码: 96-98 栏目: 出版日期: 2018-04-10
Title:
Determination of Pollen Viability of Paeonia ostii Fengdanbai
作者:
秦瑶1; 2; 董书言2; 高婷2; 鞠志新2
1.吉林农业大学,吉林 长春130118; 2.吉林农业科技学院,吉林 吉林132101
Author(s):
QIN Yao1; 2; DONG Shu-yan2; GAO Ting2; JU Zhi-xin2
1.Jilin Agricultural University,Changchun 130118,China;2.Agriculture Science and Technology College,Jilin 132101,China
关键词:
凤丹白; 花粉; 离体萌发力
Keywords:
1.Jilin Agricultural University; Changchun 130118; China; 2.Agriculture Science and Technology College; Jilin 132101; China
DOI:
10.11942/j.issn1002-2767.2018.04.0096
文献标志码:
A
摘要:
为充分利用东北地区适应性牡丹品种凤丹白进行杂交育种，采用离体萌发法研究其花粉活力，比较蔗糖及H2BO3浓度对花粉萌发率的影响，并在适宜培养浓度条件下观察花粉的萌发动态。结果表明：在凤丹白花粉离体萌发试验中硼酸与蔗糖有着重要的影响，0.05 g? L-1 H2BO3+90 g?L-1蔗糖浓度培养基为凤丹白最佳花粉培养基，花粉萌发率可达73.7%，花粉萌发出现高峰时段在离体培养的2 h后，因培养基浓度不同出现的时间也不相同。
Abstract:
In order to improve the hybrid efficiency of Paeonia ostii Fengdanbai ,a peony variety widely planted in northeast area,we used thein vitroculture method to research its pollen vigor,compared the influence of sucrose and boron acid concentrations on pollen germination rate and observed the germinating dynamics.The results showed that boron acid and sucrose played important role on the pollen germination of Fengdanbai.The maximum germination rate of pollen reached 73.7% on the culture supplied with H2BO3 0.05 g?L-1 and sucrose 90 g?L-1.Under this condition,the germination peaks in vitro culture appeared after 2 hours,and the time of peaks occurence was varied with the concentrations of H2BO3 and sucrose in culture medium.

参考文献/References:

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备注/Memo

收稿日期：2018-01-02第一作者简介：秦瑶（1993-），女，在读硕士，从事风景园林研究。E-mail：907452100@qq.com。通讯作者：鞠志新（1967-），男，博士，教授，从事园林植物种质资源与创新研究。E-mail:59393770@qq.com。

更新日期/Last Update: 2018-05-17