ZHOU Jiao-jiao,TAO Ai-en,HE Zheng-chun,et al.Changes of Accumulation of Main Secondary Metabolites in Rhizome of Paris forrestii(Takht.)H.Li.of Different Growth Years[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(03):121-125.[doi:10.11942/j.issn1002-2767.2018.03.0121]
不同生长年限长柱重楼根茎中主要次生代谢产物的累积变化
- Title:
- Changes of Accumulation of Main Secondary Metabolites in Rhizome of Paris forrestii(Takht.)H.Li.of Different Growth Years
- Keywords:
- Paris forrestii(Takht.) H.Li.; polyphyllin; secondary metabolites; HPLC; UV-Vis
- 文献标志码:
- A
- 摘要:
- 为促进长柱重楼的合理采收和资源开发利用,采用紫外分光光度计测定长柱重楼中重楼总皂苷含量;同时采用HPLC法测定不同生长年限长柱重楼中重楼皂苷I、II、VI、VII的含量,色谱柱为AgilentC-18柱(4.6 mm × 250 mm,5 μm),检测波长203 nm,柱温25 ℃,流动相为乙腈-水,流速0.9 mL?min-1,进样量5 μL;对所测数据进行主成分分析和相关性分析。结果表明:长柱重楼中主要次生代谢产物的累积量与生长年限呈非线性关系;主成分分析结果显示七年生的长柱重楼品质最佳;相关性分析结果表明总含量与重楼皂苷II、重楼皂苷I与重楼皂苷VI呈极显著正相关性。综上所述,不同生长年限长柱重楼中主要次生代谢产物的含量不同,其中重楼皂苷II总体含量偏高,长柱重楼的采收期以七年生左右为佳。
- Abstract:
- In order to promote rational collection and resources exploitation of Paris forrestii (Takht.) H.Li.,we determined the total saponins contents of P.forrestii by Ultraviolet Spectrophotometer,and the contents of polyphyllin I,II,VI and VII of P.forrestii in the different growth years by HPLC.The measured data were analyzed by principal component analysis and correlation analysis.The results showed that accumulation of main secondary metabolites in rhizome of P.forrestii showed non-linear relationships with the different growth years.And the main active components of P.forrestii in the seventh year were higher.The result of correlation analysis showed that total content and polyphyllin II, polyphyllin I and polyphyllin VI have significant positive correlation.In summary,P.forrestii(Takht.) H.Li.with different growing years differs from each other in polyphyllin .The content of polyphyllin II is high.And the best harvesting time is about 7 years.
参考文献/References:
备注/Memo
收稿日期:2017-12-19基金项目:〖JP3〗云南省科技厅面上资助项目(2013FB059);云南省大学生创新创业计划资助项目(201610679009、201610679003)。第一作者简介:周姣姣(1993-),女,在读硕士,从事中药药物化学成分研究。E-mail:ZJJ107@163.com。通讯作者:夏从龙(1974-),男,硕士,教授,硕导,从事天然药物资源调查与品质评价研究。E-mail:long7484@126.com。